Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AABM52_RS02020 Genome accession   NZ_CP150919
Coordinates   466250..468319 (+) Length   689 a.a.
NCBI ID   WP_347910158.1    Uniprot ID   -
Organism   Pseudomonas grandcourensis strain DGS24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 461250..473319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABM52_RS02000 comM 463462..464526 (+) 1065 WP_347910154.1 pilus assembly protein PilM Machinery gene
  AABM52_RS02005 - 464526..465092 (+) 567 WP_347910155.1 PilN domain-containing protein -
  AABM52_RS02010 pilO 465089..465712 (+) 624 WP_347910156.1 type 4a pilus biogenesis protein PilO -
  AABM52_RS02015 - 465709..466236 (+) 528 WP_347910157.1 pilus assembly protein PilP -
  AABM52_RS02020 pilQ 466250..468319 (+) 2070 WP_347910158.1 type IV pilus secretin PilQ Machinery gene
  AABM52_RS02025 aroK 468324..468842 (+) 519 WP_057712350.1 shikimate kinase AroK -
  AABM52_RS02030 aroB 468994..470094 (+) 1101 WP_347910159.1 3-dehydroquinate synthase -
  AABM52_RS02035 - 470105..471694 (+) 1590 WP_347910160.1 AAA family ATPase -

Sequence

Protein


Download         Length: 689 a.a.        Molecular weight: 74359.48 Da        Isoelectric Point: 5.2783

>NTDB_id=973798 AABM52_RS02020 WP_347910158.1 466250..468319(+) (pilQ) [Pseudomonas grandcourensis strain DGS24]
MNRILSTVGLSLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDAPPPVPSGYTTDSPARIALDLPGVASQLASKTRD
LGSGNARSATVVEARDRTRLIINLTQPAPYDSRIEGNNLLVVVGQGVKPSTSRPVVTATPVPARAAATAGKAIRGVDFQR
GTQGEGNVVIDLSDPSIAPDIQERDGRIIVGFAKTRLPEPLRVRLDVKDFATPVQFVNASASGDRAVISIEPGGTFDYST
YQTDNKLTVSVRPATVDELQKRNVGQQAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQA
LDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKPDERG
SITVDERTNNIIAYQTQDRLDELRRIVTQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSNGLNGS
STIGTLGSTSTSSPFVDMGVANNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQEA
SSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQS
KVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2070 bp        

>NTDB_id=973798 AABM52_RS02020 WP_347910158.1 466250..468319(+) (pilQ) [Pseudomonas grandcourensis strain DGS24]
ATGAACAGGATTCTTTCCACCGTCGGCCTTTCGCTATGGATAGCGTTGTTGTCGCCGATGGTGCAAGCGGCCAATCTCAA
GGCGCTGGATGTCGCGGCGCTGCCGGGTGACCGTGTCGAGTTGAAGTTGTCGTTCGATGCGCCGCCCCCGGTGCCCAGCG
GCTACACGACGGATTCGCCGGCCCGGATTGCCCTGGATTTGCCGGGTGTCGCCAGTCAGTTGGCCAGCAAGACTCGCGAT
CTTGGCAGTGGAAATGCCCGCAGTGCGACGGTAGTGGAAGCCCGGGACCGTACGCGTTTGATCATCAACCTGACCCAGCC
GGCCCCGTATGACTCGCGAATTGAAGGCAACAATCTGCTGGTAGTTGTCGGGCAGGGTGTCAAACCGTCGACATCCAGGC
CGGTGGTTACCGCGACGCCGGTCCCGGCACGAGCCGCGGCGACGGCAGGCAAGGCCATTCGGGGTGTGGATTTCCAGCGG
GGAACCCAGGGCGAAGGCAACGTGGTGATCGACTTGTCGGATCCGTCGATTGCGCCGGACATACAAGAGCGCGACGGCCG
GATCATCGTCGGATTTGCCAAAACCCGATTGCCCGAGCCGTTGCGCGTGCGTCTGGACGTGAAGGATTTCGCCACGCCCG
TGCAGTTCGTCAATGCCAGTGCGAGCGGTGATCGAGCCGTCATCAGTATTGAGCCCGGCGGCACGTTCGATTACTCGACC
TACCAGACTGACAATAAGCTCACGGTCAGCGTTCGCCCGGCGACGGTCGACGAGTTGCAAAAGCGCAATGTCGGGCAACA
GGCCTACAGCGGCGAGAAGCTATCGCTGAATTTCCAGGACATCGACGTGCGCTCGGTGCTGCAACTGATCGCCGATTTCA
CCAACCTCAATCTGGTGGCCAGCGACACGGTCCAGGGGGGCATTACCCTGCGCCTGCAGAACGTGCCGTGGGATCAGGCG
CTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGGTCGGCAATGTCCTGCTGGTTGCGCCGGCCGATGAAAT
TGCTGCCCGTGAGCGTCAGGAGCTCGAATCGCAGAAGCAGATCGCCGATCTGGCGCCGTTGCGTCGGGAGCTGTTGCAGG
TCAATTACGCCAAGGCGGCGGACATCGCCAAGCTCTTTCAGTCGGTGACCAGTGCCGAGGCGAAACCCGACGAACGGGGG
TCGATCACCGTTGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGACCGGCTGGACGAACTGCGCAGGATCGT
CACGCAACTGGATATCCCGGTACGTCAGGTGATGATCGAGGCGCGCATTGTCGAAGCCAACGTCGACTATGACAAAAGCC
TCGGGGTGCGCTGGGGCGGCTCGATACAGAACAAGGGCAACTGGAATACCTCCGGTGTCAGCAACGGACTCAATGGATCG
TCGACCATCGGCACGCTCGGCAGTACCAGCACCAGCTCACCGTTCGTCGACATGGGCGTAGCCAACAACACCTCGGGAAT
CGGCATCGCGTTCATCACCGATAACGTCTTGCTGGACCTGGAGCTGACGGCCATGGAAAAAACCGGCAACGGGGAAATCG
TCTCGCAGCCCAAGGTGGTCACGTCCGACAAGGAAACCGCGAAGATCCTCAAGGGCACCGAGATTCCCTATCAGGAAGCC
AGTTCCAGCGGCGCTACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAGGTGACGCCGCAGATCACGCCGGACAA
CCGGATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTATCTGAACAAGGTCCAGGATGTACCGCCCATCAAGA
AGAACGAAGTCAACGCCAAGGTCCTGGTCAACGATGGCGAGACCATCGTCATTGGCGGCGTTTTCTCAAATACTCAGAGC
AAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTTTCGGAGAA
AAAATCCGAGCTGTTGGTATTCCTCACTCCGCGTATCATGAATAATCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-121)

Secretin and TonB N terminus short domain (STN)

(297-343)

Bacterial type II and III secretion system protein (Secretin)

(524-680)

Bacterial type II/III secretion system short domain (Secretin_N)

(371-436)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.369

100

0.742
  comQ Acinetobacter baylyi ADP1

41.304

100

0.414
  pilQ Acinetobacter baumannii strain A118

38.019

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.019

100

0.412
  pilQ Legionella pneumophila strain Lp02

38.757

98.113

0.38
  pilQ Legionella pneumophila strain ERS1305867

38.609

98.113

0.379

Multiple sequence alignment