Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   WOC54_RS12235 Genome accession   NZ_CP150747
Coordinates   2406635..2407132 (+) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain TUM22701     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2401635..2412132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WOC54_RS12195 (WOC54_12225) - 2401679..2402665 (+) 987 WP_000161311.1 ROK family glucokinase -
  WOC54_RS12200 (WOC54_12230) - 2402665..2402994 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  WOC54_RS12205 (WOC54_12235) - 2402991..2403614 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  WOC54_RS12210 (WOC54_12240) comGA 2403666..2404640 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  WOC54_RS12215 (WOC54_12245) comGB 2404612..2405682 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  WOC54_RS12220 (WOC54_12250) comGC 2405696..2406007 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  WOC54_RS12225 (WOC54_12255) comGD 2405985..2406431 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  WOC54_RS12230 (WOC54_12260) comGE 2406418..2406717 (+) 300 WP_000844410.1 hypothetical protein Machinery gene
  WOC54_RS12235 (WOC54_12265) comGF 2406635..2407132 (+) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  WOC54_RS12240 (WOC54_12270) - 2407229..2407375 (+) 147 WP_001792109.1 hypothetical protein -
  WOC54_RS12245 (WOC54_12275) - 2407365..2407889 (+) 525 WP_001015120.1 shikimate kinase -
  WOC54_RS12250 (WOC54_12280) gcvT 2408048..2409139 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  WOC54_RS12255 (WOC54_12285) gcvPA 2409159..2410505 (+) 1347 WP_341262150.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  WOC54_RS12260 (WOC54_12290) gcvPB 2410498..2411970 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=971724 WOC54_RS12235 WP_001788897.1 2406635..2407132(+) (comGF) [Staphylococcus aureus strain TUM22701]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=971724 WOC54_RS12235 WP_001788897.1 2406635..2407132(+) (comGF) [Staphylococcus aureus strain TUM22701]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment