Detailed information NTG0970804

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACLII2_RS13575
• Genome accession: NZ_CP178213
• Coordinates: 2570937..2573354 (-)
• Length: 805 a.a.
• NCBI ID: WP_003232079.1
• Uniprot ID: P94461
• Organism: Bacillus subtilis subsp. subtilis strain BM107
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2565937..2578354

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLII2_RS13550	prpC	2566274..2567038 (-)	765	WP_003232064.1	protein-serine/threonine phosphatase PrpC	-
ACLII2_RS13555	rlmN	2567045..2568136 (-)	1092	WP_003232066.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
ACLII2_RS13560	rsmB	2568140..2569483 (-)	1344	WP_003232068.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
ACLII2_RS13565	fmt	2569470..2570423 (-)	954	WP_003232070.1	methionyl-tRNA formyltransferase	-
ACLII2_RS13570	def	2570428..2570910 (-)	483	WP_003232077.1	peptide deformylase	-
ACLII2_RS13575	priA	2570937..2573354 (-)	2418	WP_003232079.1	primosomal protein N'	Machinery gene
ACLII2_RS13580	coaBC	2573351..2574571 (-)	1221	WP_009967219.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
ACLII2_RS13585	rpoZ	2574652..2574855 (-)	204	WP_003221520.1	DNA-directed RNA polymerase subunit omega	-
ACLII2_RS13590	gmk	2574859..2575473 (-)	615	WP_003232083.1	guanylate kinase	-
ACLII2_RS13595	remA	2575481..2575750 (-)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
ACLII2_RS13600	endC	2575827..2576702 (-)	876	WP_009967215.1	YicC/YloC family endoribonuclease	-

Sequence

Protein

• Length: 805 a.a.
• Molecular weight: 91353.42 Da
• Isoelectric Point: 8.4336

>NTDB_id=970804 ACLII2_RS13575 WP_003232079.1 2570937..2573354(-) (priA) [Bacillus subtilis subsp. subtilis strain BM107]
MNFAEVIVDVSTKNIDRPFDYKIPDHLKGMIKTGMRVIVPFGPRKIQGFVTAVKEASDLSGKSVKEVEDLLDLTPVLTEE
LMILSSWLSDKTLSFKITALQAMLPAALKAKYEKELKIAHGADLPPQVERLFSETKTLLYSDIPDHETLKLIQRHVQKGD
IDVTYKVAQKTNKKMVRHIQANASKEELAKQAEGLSRQAAKQQAILHFLISEPEGVKIPAAELCKKTDTSSATIKTLIQK
GLLKESYEEVYRDPYQDKMFKKTEPLPLTDEQRAAFEPIRETLDSDEHKVFLLHGVTGSGKTEIYLQSIEKVLAKGKEAI
VLVPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQ
EEMPRYHAKEVAIKRAEHHSCPVVLGSATPTLESYARAQKGVYELLSLKHRVNHRVMPEVSLVDMREELRNGNRSMFSVE
LMEKLEETIAKGEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPHTCPECASEHIRF
FGTGTQRVEEELTKVLPSARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIP
DFRSAEKTFQLLTQVSGRAGRHEKPGHVIIQTYTPSHYSIQLTKTHDYETFYQHEMAHRREQSYPPYYYLALVTVSHEEV
AKAAVTAEKIAHFLKANCGADTKILGPSASPIARIKDRYRYQCVIKYKQETQLSALLKKILEHYKREIEQKHVMISIDMN
PYMMM

Nucleotide

• Length: 2418 bp

>NTDB_id=970804 ACLII2_RS13575 WP_003232079.1 2570937..2573354(-) (priA) [Bacillus subtilis subsp. subtilis strain BM107]
ATGAATTTTGCAGAAGTCATCGTTGATGTCAGCACCAAAAATATAGACAGGCCTTTTGATTATAAAATCCCAGACCATCT
GAAGGGCATGATCAAAACGGGGATGCGGGTCATTGTTCCGTTTGGCCCCCGTAAGATTCAAGGGTTTGTGACAGCAGTCA
AAGAAGCATCTGACCTATCGGGAAAATCTGTCAAGGAAGTAGAGGATTTATTAGATCTTACACCTGTTCTGACAGAGGAG
CTTATGATTCTGTCATCATGGCTGTCAGACAAAACGCTGTCTTTTAAAATAACGGCGCTTCAAGCAATGCTGCCTGCCGC
CTTAAAGGCGAAATATGAAAAAGAATTAAAAATTGCACACGGAGCCGATCTTCCGCCGCAAGTGGAACGGCTGTTTTCTG
AAACAAAGACGCTGCTGTATTCAGATATACCCGATCATGAGACGCTGAAACTGATTCAAAGGCATGTTCAAAAAGGGGAC
ATTGACGTGACGTATAAAGTCGCGCAAAAAACCAATAAAAAAATGGTCAGGCATATTCAGGCAAATGCGAGTAAAGAAGA
GCTTGCTAAGCAGGCTGAGGGATTGTCCCGCCAAGCAGCAAAACAGCAGGCGATTCTTCACTTTCTCATCTCTGAGCCGG
AAGGTGTGAAAATTCCTGCGGCGGAATTGTGCAAAAAAACGGACACGAGCTCTGCAACCATCAAAACCCTTATCCAAAAA
GGGCTTTTGAAGGAAAGCTATGAAGAGGTTTACAGAGATCCCTATCAGGACAAAATGTTCAAAAAAACAGAGCCCCTGCC
GCTGACAGACGAACAGAGGGCTGCCTTCGAGCCCATACGCGAGACATTGGACAGCGATGAGCATAAAGTGTTCCTCCTTC
ACGGCGTGACAGGAAGCGGAAAAACCGAAATTTATCTGCAATCAATTGAAAAAGTACTGGCGAAAGGAAAAGAAGCCATC
GTCCTCGTACCGGAAATATCGCTGACGCCGCAAATGGTCAACCGCTTCAAGGGCCGCTTCGGCTCCCAGGTGGCAGTCAT
GCACAGCGGTTTATCCACAGGAGAAAAATATGACGAGTGGCGTAAAATCCACCGGAAGGAAGTACGGCTTGTCGTCGGTG
CCAGATCCGCCATTTTTGCTCCGTTTGAAAATCTCGGAATGATAATCATCGATGAAGAGCACGAGTCTTCCTATAAACAG
GAAGAAATGCCGCGCTATCATGCGAAAGAGGTGGCAATCAAGCGGGCTGAGCATCACAGCTGCCCGGTTGTATTAGGGAG
TGCTACGCCGACATTAGAATCATATGCTAGAGCGCAAAAGGGCGTATACGAGCTTCTTTCACTGAAGCACCGTGTTAACC
ATCGGGTGATGCCCGAGGTTTCGCTTGTCGATATGAGAGAGGAGCTTAGAAACGGCAATCGCTCGATGTTTTCAGTTGAA
TTGATGGAAAAGCTTGAAGAAACGATTGCCAAGGGTGAGCAGGCGGTGCTGTTTTTAAATAAGAGGGGCTACTCTTCCTT
TGTGATGTGCAGGGATTGCGGCTATGTCCCGCAATGCCCGCACTGCGACATTTCCATGACGTATCATCGTTACGGCCAAA
GGCTGAAATGCCATTATTGCGGACATGAAGAGCCTGTTCCGCACACTTGTCCTGAATGCGCAAGCGAGCATATCCGCTTT
TTCGGAACGGGAACACAGCGAGTGGAGGAAGAGCTGACAAAAGTGCTGCCAAGTGCGAGAGTGATTCGGATGGATGTTGA
CACGACATCACGGAAAGGCGCCCATGAAAAATTACTGTCAGCTTTCGGAGAAGGAAAAGCGGATATTCTTCTCGGAACGC
AAATGATCGCGAAAGGGCTTGATTTTCCGAATGTCACGCTTGTCGGAGTGTTAAGTGCTGATACAACACTTCATATTCCT
GATTTCAGATCGGCTGAAAAAACATTTCAGCTGTTAACGCAAGTCAGCGGCCGGGCAGGCAGGCATGAAAAGCCCGGCCA
TGTCATCATTCAAACGTATACACCGTCTCATTACAGTATCCAGCTGACGAAAACACACGATTATGAAACGTTCTATCAGC
ATGAAATGGCGCACAGGAGAGAGCAATCCTATCCGCCATACTACTATTTGGCTCTTGTGACTGTTTCTCATGAGGAAGTG
GCGAAAGCAGCGGTTACAGCTGAAAAAATCGCTCATTTTTTGAAAGCAAACTGCGGAGCTGACACGAAAATTCTCGGACC
AAGCGCGTCGCCGATAGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAAACAGGAAACCCAGTTAT
CCGCTTTATTGAAGAAGATACTGGAACATTATAAACGCGAAATTGAACAAAAACATGTAATGATTTCAATTGATATGAAT
CCTTATATGATGATGTAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (706-801)

• Helicase conserved C-terminal domain (Helicase_C) (565-662)

• Type III restriction enzyme, res subunit (ResIII) (267-431)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (519-545)

3D structure

• AlphaFold DB: P94461

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	100	100	1