Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACLII2_RS08320 Genome accession   NZ_CP178213
Coordinates   1576295..1576912 (+) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   P39694
Organism   Bacillus subtilis subsp. subtilis strain BM107     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1571295..1581912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLII2_RS08285 aroE 1571953..1572795 (+) 843 WP_003229967.1 shikimate dehydrogenase -
  ACLII2_RS08290 yhbY 1572789..1573079 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  ACLII2_RS08295 nadD 1573091..1573660 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  ACLII2_RS08300 yqeK 1573650..1574210 (+) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACLII2_RS08305 rsfS 1574228..1574584 (+) 357 WP_003229971.1 ribosome silencing factor -
  ACLII2_RS08310 yqeM 1574581..1575324 (+) 744 WP_003229973.1 class I SAM-dependent DNA methyltransferase -
  ACLII2_RS08315 comER 1575390..1576211 (-) 822 WP_004398597.1 late competence protein ComER -
  ACLII2_RS08320 comEA 1576295..1576912 (+) 618 WP_004398514.1 competence protein ComEA Machinery gene
  ACLII2_RS08325 comEB 1576979..1577548 (+) 570 WP_003229978.1 ComE operon protein 2 -
  ACLII2_RS08330 comEC 1577552..1579882 (+) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACLII2_RS08335 yqzM 1579922..1580056 (-) 135 WP_003229983.1 YqzM family protein -
  ACLII2_RS08340 - 1580097..1580246 (+) 150 WP_003229985.1 hypothetical protein -
  ACLII2_RS08345 holA 1580286..1581329 (+) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  ACLII2_RS08350 rpsT 1581344..1581610 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=970767 ACLII2_RS08320 WP_004398514.1 1576295..1576912(+) (comEA) [Bacillus subtilis subsp. subtilis strain BM107]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=970767 ACLII2_RS08320 WP_004398514.1 1576295..1576912(+) (comEA) [Bacillus subtilis subsp. subtilis strain BM107]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8DFK

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39