Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WIF35_RS11565 Genome accession   NZ_CP150631
Coordinates   2366478..2367941 (-) Length   487 a.a.
NCBI ID   WP_412478366.1    Uniprot ID   -
Organism   Azonexus sp. IMCC34839     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2361478..2372941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WIF35_RS11535 ttcA 2362515..2363378 (+) 864 WP_412478360.1 tRNA 2-thiocytidine(32) synthetase TtcA -
  WIF35_RS11540 - 2363442..2364338 (+) 897 WP_412478361.1 FHA domain-containing protein -
  WIF35_RS11545 - 2364390..2364647 (-) 258 WP_412478362.1 hypothetical protein -
  WIF35_RS11550 hprK 2364698..2365633 (-) 936 WP_412478363.1 HPr(Ser) kinase/phosphatase -
  WIF35_RS11555 ptsN 2365641..2366087 (-) 447 WP_412478364.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WIF35_RS11560 hpf 2366145..2366465 (-) 321 WP_412478365.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WIF35_RS11565 rpoN/rpoN1 2366478..2367941 (-) 1464 WP_412478366.1 RNA polymerase factor sigma-54 Machinery gene
  WIF35_RS11570 lptB 2367970..2368701 (-) 732 WP_412478367.1 LPS export ABC transporter ATP-binding protein -
  WIF35_RS11575 recX 2368698..2369129 (-) 432 WP_412478368.1 recombination regulator RecX -
  WIF35_RS11580 recA 2369122..2370153 (-) 1032 WP_412478369.1 recombinase RecA Machinery gene
  WIF35_RS11585 - 2370343..2370543 (+) 201 WP_412478370.1 hypothetical protein -
  WIF35_RS11590 - 2370540..2371187 (+) 648 WP_412478371.1 RluA family pseudouridine synthase -
  WIF35_RS11595 - 2371258..2372754 (+) 1497 WP_412478372.1 sodium:solute symporter family protein -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 55314.37 Da        Isoelectric Point: 4.8255

>NTDB_id=970727 WIF35_RS11565 WP_412478366.1 2366478..2367941(-) (rpoN/rpoN1) [Azonexus sp. IMCC34839]
MKPALQLKLSQHLALTPQLQQSIKLLQLSTVEMQQEIERYLLENPMLEREDDNGGESFAGAQQFDSPRTNENERERESEE
RTPSEDREQEIPSSPSEVDDDRWTSDAGTFTGAGRDEDDDSDAHDIHAANVSLREHLGWQLGMTQLTERDRSLVRFLIEA
LDDDGYLSTPLLELWETLPPEYEIELEELEIALRHIQNFDPTGIGARNLQECLLLQLKAEEESEERDLAMVIVDKHLELL
AARDFTKIKRLAGCDDAELRDAQALITSLNPRPGAGYAQLEARYITPDVIVKKLKGKWTAYINPDAYPRLRINRLYAEIL
AKQRRGNGNLSTQLQEARWLIKNVQQRFETIHRVTQAIVDRQRQFFEHGEVAMRPLVLREIADILGLHESTVSRVTSQKY
MATPRGIFELKYFFGSHVSTDTGGACSATAIRALIKQLIAAEDGKKPLSDSQLSEILGQQGIVVARRTVAKYRESLNIPP
VNLRKTL

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=970727 WIF35_RS11565 WP_412478366.1 2366478..2367941(-) (rpoN/rpoN1) [Azonexus sp. IMCC34839]
ATGAAGCCCGCTCTCCAACTCAAACTCTCCCAGCACCTGGCGCTGACGCCTCAGTTGCAGCAGTCGATCAAGCTCCTGCA
GTTGTCGACGGTGGAGATGCAGCAGGAAATCGAGCGCTATCTGCTCGAAAATCCCATGCTGGAGCGGGAAGACGACAACG
GCGGCGAGTCTTTCGCCGGGGCGCAGCAGTTCGACTCGCCGCGCACGAACGAAAACGAGCGTGAGCGGGAAAGCGAAGAG
CGCACCCCGAGCGAGGATCGCGAGCAGGAAATTCCCTCCTCGCCGTCCGAAGTCGACGACGATCGCTGGACTTCGGATGC
CGGTACGTTCACCGGTGCCGGGCGCGACGAAGACGACGACAGCGATGCCCACGACATTCATGCTGCCAACGTCAGCCTGC
GCGAGCATCTGGGCTGGCAACTCGGCATGACCCAGCTTACCGAGCGTGACCGTAGTTTGGTCCGCTTCCTGATCGAAGCA
CTCGATGACGACGGCTATCTGTCGACTCCCTTGCTGGAACTGTGGGAAACCCTGCCGCCGGAATATGAGATCGAGCTGGA
GGAGCTGGAAATCGCCTTGCGCCATATCCAGAACTTCGACCCGACCGGCATCGGTGCACGCAACCTGCAGGAATGCTTGC
TGCTGCAACTTAAGGCGGAAGAGGAAAGCGAAGAGCGCGATCTGGCCATGGTCATTGTCGACAAGCACCTCGAATTGCTT
GCCGCTCGCGACTTCACCAAAATCAAGCGTTTGGCCGGCTGCGACGATGCCGAGCTGAGGGATGCGCAAGCGCTGATCAC
CAGCCTCAATCCACGCCCCGGTGCGGGTTATGCGCAACTGGAAGCCCGTTACATCACGCCCGACGTAATCGTGAAGAAGC
TGAAGGGCAAGTGGACGGCCTACATCAATCCGGATGCCTATCCGCGCCTGCGCATCAACCGCTTGTATGCGGAAATCCTC
GCCAAGCAGCGGCGCGGCAATGGCAACCTGTCGACCCAATTGCAGGAAGCGCGCTGGCTGATCAAGAATGTCCAGCAGCG
TTTCGAGACCATTCATCGCGTCACGCAAGCCATCGTCGACCGTCAGCGCCAGTTCTTCGAACATGGCGAAGTGGCGATGC
GGCCCCTCGTCCTGCGCGAAATTGCCGACATTCTCGGCTTGCACGAATCGACCGTGTCGCGGGTGACCAGCCAGAAGTAC
ATGGCGACACCGCGCGGCATTTTCGAACTGAAATACTTCTTTGGCAGCCATGTTTCCACCGATACCGGTGGCGCCTGTTC
TGCCACGGCCATCCGGGCGTTGATCAAGCAATTGATTGCGGCTGAGGACGGCAAGAAGCCCTTGTCGGATAGCCAATTAT
CCGAAATACTAGGCCAGCAGGGTATTGTTGTTGCGCGCCGAACGGTGGCCAAATACCGTGAATCGCTCAACATCCCGCCG
GTCAATTTACGTAAGACTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(330-485)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(125-316)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

55.378

100

0.571

Multiple sequence alignment