Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   WHC13_RS13880 Genome accession   NZ_CP150487
Coordinates   2640113..2640730 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain JNF2     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2635113..2645730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHC13_RS13850 (WHC13_13850) rpsT 2635415..2635681 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  WHC13_RS13855 (WHC13_13855) holA 2635696..2636739 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  WHC13_RS13860 (WHC13_13860) - 2636779..2636928 (-) 150 WP_003229985.1 hypothetical protein -
  WHC13_RS13865 (WHC13_13865) yqzM 2636969..2637103 (+) 135 WP_003229983.1 YqzM family protein -
  WHC13_RS13870 (WHC13_13870) comEC 2637143..2639473 (-) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  WHC13_RS13875 (WHC13_13875) comEB 2639477..2640046 (-) 570 WP_003229978.1 ComE operon protein 2 -
  WHC13_RS13880 (WHC13_13880) comEA 2640113..2640730 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  WHC13_RS13885 (WHC13_13885) comER 2640814..2641635 (+) 822 WP_004398597.1 late competence protein ComER -
  WHC13_RS13890 (WHC13_13890) yqeM 2641701..2642444 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  WHC13_RS13895 (WHC13_13895) rsfS 2642441..2642797 (-) 357 WP_003229971.1 ribosome silencing factor -
  WHC13_RS13900 (WHC13_13900) yqeK 2642815..2643375 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  WHC13_RS13905 (WHC13_13905) nadD 2643365..2643934 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  WHC13_RS13910 (WHC13_13910) yhbY 2643946..2644236 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  WHC13_RS13915 (WHC13_13915) aroE 2644230..2645072 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=970602 WHC13_RS13880 WP_004398514.1 2640113..2640730(-) (comEA) [Bacillus subtilis strain JNF2]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=970602 WHC13_RS13880 WP_004398514.1 2640113..2640730(-) (comEA) [Bacillus subtilis strain JNF2]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment