Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   ACLF6N_RS09075 Genome accession   NZ_CP177279
Coordinates   1702617..1704563 (+) Length   648 a.a.
NCBI ID   WP_412052570.1    Uniprot ID   -
Organism   Bacillus subtilis strain KFRI-P74     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 1697617..1709563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLF6N_RS09050 (ACLF6N_09050) def 1697987..1698469 (+) 483 WP_014479759.1 peptide deformylase -
  ACLF6N_RS09055 (ACLF6N_09055) fmt 1698474..1699427 (+) 954 WP_129133969.1 methionyl-tRNA formyltransferase -
  ACLF6N_RS09060 (ACLF6N_09060) rsmB 1699414..1700757 (+) 1344 WP_015252111.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  ACLF6N_RS09065 (ACLF6N_09065) rlmN 1700761..1701852 (+) 1092 WP_069837503.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACLF6N_RS09070 (ACLF6N_09070) prpC 1701859..1702623 (+) 765 WP_043857480.1 protein-serine/threonine phosphatase PrpC -
  ACLF6N_RS09075 (ACLF6N_09075) stkP 1702617..1704563 (+) 1947 WP_412052570.1 serine/threonine protein kinase PrkC Regulator
  ACLF6N_RS09080 (ACLF6N_09080) rsgA 1704578..1705474 (+) 897 WP_043857482.1 ribosome small subunit-dependent GTPase A -
  ACLF6N_RS09085 (ACLF6N_09085) rpe 1705479..1706132 (+) 654 WP_003232058.1 ribulose-phosphate 3-epimerase -
  ACLF6N_RS09090 (ACLF6N_09090) thiN 1706205..1706849 (+) 645 WP_014479765.1 thiamine diphosphokinase -
  ACLF6N_RS09095 (ACLF6N_09095) spoVM 1706921..1707001 (+) 81 WP_003221545.1 stage V sporulation protein SpoVM -
  ACLF6N_RS09100 (ACLF6N_09100) rpmB 1707074..1707262 (-) 189 WP_003221548.1 50S ribosomal protein L28 -
  ACLF6N_RS09105 (ACLF6N_09105) yloU 1707539..1707901 (+) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -

Sequence

Protein


Download         Length: 648 a.a.        Molecular weight: 71962.58 Da        Isoelectric Point: 4.7900

>NTDB_id=970265 ACLF6N_RS09075 WP_412052570.1 1702617..1704563(+) (stkP) [Bacillus subtilis strain KFRI-P74]
MLIGKRISGRYQILRVIGGGGMANVYLAEDIILDREVAIKILRFDYANDNEFIRRFRREAQSASSLDHPNIVSIYDLGEE
DDIYYIVMEYVEGMTLKEYITANGPLHPKEALNIMEQIVSAIAHAHQNQIVHRDIKPHNILIDHMGNIKVTDFGIATALS
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGRIPFDGESAVSIALKHLQAETPSAKRWNPSVPQS
VENIILKATAKDPFHRYETAEDMEADIKTAFDADRLNEKRFTIQEDEEMTKAIPIIKDEELAKAAGEKEAEVTTAQENKT
KKNGKRKKWPWVLLTICLVFITAGILAVTVFPSLFMPKDVKIPDVSGMEYEKAAGHLEKEGLQVDSEVLEISDEKIEEGL
MVKTDPKADTTVKEGATVTLYKSTGKAKTEIDDVTGQTVDQAKKALKDQGFKHVTVNEVNDEKNAGTVIDQNPSAGTELV
PSEDQVKLTVSIGPEDITLRDLKTYSKEAASGYLEDNGLKLVEKEAYSDDVPEGQVVKQKPAAGTAVKPGNEVEVTFSLG
PEKKPAKTVKEKVKIPYEPENEGDELQVQIAVDDADHSISDTYEEFKIKEPTERTIELKIEPGQKGYYQVMVNNKVVSYK
TIEYPKDE

Nucleotide


Download         Length: 1947 bp        

>NTDB_id=970265 ACLF6N_RS09075 WP_412052570.1 1702617..1704563(+) (stkP) [Bacillus subtilis strain KFRI-P74]
GTGCTAATCGGCAAGCGGATCAGCGGGCGTTACCAAATTCTCCGCGTTATAGGCGGCGGGGGAATGGCCAACGTGTATTT
AGCTGAGGATATCATTCTAGACCGTGAAGTCGCAATTAAAATCCTGCGGTTTGACTATGCAAATGACAATGAATTTATCA
GACGTTTCCGCAGAGAAGCCCAATCCGCATCAAGCCTCGATCACCCGAATATTGTCAGCATTTACGATTTGGGCGAGGAA
GATGATATTTATTATATTGTCATGGAATACGTTGAAGGCATGACGCTTAAAGAATACATAACAGCAAATGGGCCGCTTCA
CCCTAAAGAAGCGCTGAACATCATGGAGCAAATTGTCTCAGCCATCGCTCATGCCCATCAAAACCAGATTGTTCACAGAG
ACATCAAGCCGCATAACATTTTGATTGATCATATGGGAAATATCAAAGTGACGGATTTTGGAATTGCGACGGCACTAAGT
TCGACCACAATCACCCATACCAATTCAGTTCTGGGCTCGGTCCATTACTTATCACCTGAACAGGCCCGGGGCGGCTTAGC
CACAAAAAAATCAGATATTTATGCGCTTGGAATCGTTCTATTTGAGCTTTTAACCGGCCGTATTCCGTTTGATGGAGAGT
CAGCAGTCAGCATCGCCTTGAAGCATCTTCAAGCGGAAACTCCTTCGGCAAAAAGGTGGAATCCATCGGTCCCCCAAAGC
GTTGAAAACATCATACTCAAGGCAACTGCCAAAGATCCGTTTCATCGCTATGAAACGGCTGAAGACATGGAAGCAGACAT
AAAAACAGCTTTTGATGCCGACAGACTCAATGAAAAGAGATTTACGATTCAAGAAGATGAAGAAATGACAAAAGCGATAC
CTATCATTAAAGATGAAGAACTCGCTAAAGCTGCTGGCGAAAAAGAAGCTGAAGTGACAACCGCACAAGAAAACAAAACA
AAGAAGAACGGCAAAAGAAAAAAGTGGCCGTGGGTTTTGCTCACGATATGCCTTGTTTTTATCACAGCTGGAATTCTTGC
TGTCACTGTTTTTCCGTCGCTTTTCATGCCTAAGGATGTCAAAATACCTGATGTCTCCGGAATGGAATACGAAAAAGCCG
CAGGGCACTTGGAAAAAGAAGGTTTACAGGTTGATTCCGAGGTGTTGGAAATCTCAGATGAAAAAATTGAAGAGGGCCTG
ATGGTAAAAACGGACCCTAAAGCGGATACAACAGTCAAAGAAGGCGCCACGGTCACTCTTTATAAAAGCACCGGAAAGGC
AAAAACGGAGATCGATGATGTGACAGGCCAAACGGTCGACCAGGCAAAAAAAGCGTTGAAGGACCAAGGGTTTAAACATG
TAACGGTAAATGAAGTGAATGACGAGAAAAACGCGGGAACTGTCATTGACCAAAATCCTTCAGCAGGGACTGAGCTTGTC
CCGAGTGAAGATCAAGTCAAACTTACAGTCAGTATCGGACCCGAAGACATTACACTTAGAGACTTGAAAACCTACAGTAA
AGAAGCGGCGTCTGGATATCTGGAAGACAACGGATTGAAGCTTGTAGAAAAAGAAGCATACTCAGATGATGTTCCAGAAG
GACAGGTTGTCAAACAAAAACCAGCAGCAGGTACGGCAGTAAAGCCGGGAAACGAAGTTGAAGTGACATTCTCTCTCGGA
CCAGAGAAAAAACCTGCGAAAACAGTGAAAGAAAAGGTCAAGATCCCCTATGAACCAGAAAATGAAGGGGACGAGCTTCA
AGTGCAAATCGCGGTTGACGATGCTGATCACAGCATCTCTGACACTTACGAAGAATTTAAGATAAAAGAGCCGACTGAAC
GAACGATCGAACTAAAGATTGAACCCGGCCAAAAAGGGTACTATCAAGTAATGGTAAACAATAAAGTTGTCAGCTACAAA
ACCATTGAGTATCCGAAAGATGAATAA

Domains

Predicted by InterProScan.

Protein kinase domain (Pkinase)

(11-261)

PASTA domain (PASTA)

(360-423)

PASTA domain (PASTA)

(498-558)

PASTA domain (PASTA)

(430-491)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

40.517

89.506

0.363
  stkP Streptococcus pneumoniae D39

40.345

89.506

0.361
  stkP Streptococcus pneumoniae R6

40.345

89.506

0.361

Multiple sequence alignment