Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MHI53_RS15810 Genome accession   NZ_CP150304
Coordinates   3289226..3290296 (-) Length   356 a.a.
NCBI ID   WP_340371711.1    Uniprot ID   -
Organism   Peribacillus sp. FSL E2-0218     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3284226..3295296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI53_RS15770 (MHI53_15770) - 3284359..3285156 (+) 798 WP_340371710.1 YqhG family protein -
  MHI53_RS15775 (MHI53_15775) - 3285944..3286129 (-) 186 WP_061142003.1 YqzE family protein -
  MHI53_RS15780 (MHI53_15780) comGG 3286233..3286619 (-) 387 WP_061142002.1 competence type IV pilus minor pilin ComGG -
  MHI53_RS15785 (MHI53_15785) comGF 3286612..3287091 (-) 480 WP_185113070.1 competence type IV pilus minor pilin ComGF -
  MHI53_RS15790 (MHI53_15790) - 3287039..3287377 (-) 339 WP_061142000.1 hypothetical protein -
  MHI53_RS15795 (MHI53_15795) comGD 3287364..3287774 (-) 411 WP_061141999.1 competence type IV pilus minor pilin ComGD -
  MHI53_RS15800 (MHI53_15800) comGC 3287815..3288123 (-) 309 WP_061141998.1 competence type IV pilus major pilin ComGC -
  MHI53_RS15805 (MHI53_15805) comGB 3288202..3289233 (-) 1032 WP_061141997.1 competence type IV pilus assembly protein ComGB -
  MHI53_RS15810 (MHI53_15810) comGA 3289226..3290296 (-) 1071 WP_340371711.1 competence type IV pilus ATPase ComGA Machinery gene
  MHI53_RS15815 (MHI53_15815) - 3290401..3290781 (-) 381 WP_061141995.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHI53_RS15820 (MHI53_15820) - 3290993..3291694 (+) 702 WP_061141994.1 helix-turn-helix domain-containing protein -
  MHI53_RS15825 (MHI53_15825) - 3291779..3292021 (+) 243 WP_061141993.1 DUF2626 domain-containing protein -
  MHI53_RS15830 (MHI53_15830) - 3292081..3293178 (-) 1098 WP_185113069.1 SAM-dependent methyltransferase -
  MHI53_RS15835 (MHI53_15835) - 3293382..3294008 (-) 627 WP_061142098.1 MBL fold metallo-hydrolase -
  MHI53_RS15840 (MHI53_15840) - 3294237..3294410 (+) 174 WP_064505148.1 DUF2759 domain-containing protein -
  MHI53_RS15845 (MHI53_15845) - 3294715..3294963 (-) 249 WP_340371712.1 hypothetical protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39618.03 Da        Isoelectric Point: 8.4749

>NTDB_id=970098 MHI53_RS15810 WP_340371711.1 3289226..3290296(-) (comGA) [Peribacillus sp. FSL E2-0218]
MISIEKTAEKILTRAIRLSATDIHIFFRREGPLIQFRIDNKLVPQETISFFEAERLIAHLKFLAAMDIGEKRRPQSGAIT
MNLADQVVGLRLSTLPTAYLESLVIRLIPQQNILPLEQLSLFPNTAQKLIALLKHSHGMLIFTGPTGSGKTTTLYSLLHH
ARVMLNRNIITLEDPIENVSEKVLQVQINEKAGITYSVGLKAVLRHDPDVIMVGEIRDSETAKIAVRAALTGHLILTTMH
TRDAQGAISRLLEFGASLLEIEQSLVGVTAQRLVELRCLSCNGDCPSACERTDRTKRASVYELLFGKSLADLFQTIKDEK
GGASVSYRRLKDEIGKAVAMGFVGTQEYDRLVYRDD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=970098 MHI53_RS15810 WP_340371711.1 3289226..3290296(-) (comGA) [Peribacillus sp. FSL E2-0218]
TTGATATCGATAGAAAAAACGGCAGAAAAAATCCTGACTCGTGCCATTCGGTTATCGGCGACTGACATCCATATTTTTTT
TCGCAGGGAGGGGCCGCTCATCCAATTCAGGATAGACAATAAGCTCGTACCGCAAGAAACAATATCATTCTTTGAAGCTG
AAAGGCTTATAGCCCACTTGAAGTTCCTTGCCGCAATGGATATAGGCGAAAAAAGGAGGCCGCAAAGTGGCGCCATCACC
ATGAATTTAGCTGACCAAGTGGTAGGACTTCGCCTCTCCACACTCCCCACTGCCTATCTCGAAAGTCTGGTCATCCGCTT
GATACCCCAACAGAATATCCTTCCGCTAGAACAATTATCCTTATTCCCGAATACCGCCCAAAAATTGATCGCGCTCCTGA
AGCACTCCCATGGCATGCTCATTTTTACCGGCCCCACCGGCAGCGGGAAAACGACTACCCTATATTCCCTGCTTCATCAT
GCACGTGTGATGCTCAATCGAAATATCATTACACTTGAAGATCCGATTGAAAATGTGTCCGAAAAGGTGCTGCAAGTCCA
AATCAATGAAAAGGCGGGCATTACCTATTCCGTCGGCCTAAAAGCTGTGCTTAGGCATGACCCGGACGTGATCATGGTGG
GGGAAATCAGGGATTCGGAAACGGCGAAAATCGCGGTGCGTGCCGCATTGACCGGACATTTGATCCTGACGACGATGCAT
ACAAGGGATGCACAAGGGGCCATCTCCAGGCTGCTTGAATTTGGTGCAAGCCTGCTTGAAATAGAACAAAGCTTGGTTGG
CGTGACAGCTCAGCGTCTGGTTGAACTTCGCTGCCTTTCGTGTAACGGGGATTGTCCATCTGCTTGCGAACGGACGGATA
GGACGAAAAGGGCGAGTGTGTATGAATTGCTTTTTGGGAAAAGCTTGGCCGACCTTTTCCAAACGATTAAGGATGAAAAG
GGCGGCGCGTCTGTCAGCTATCGTAGATTAAAGGATGAAATTGGTAAAGCGGTTGCCATGGGTTTTGTCGGTACACAAGA
ATACGATCGGCTGGTATATCGTGATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.303

100

0.565
  pilB Haemophilus influenzae 86-028NP

38.177

98.596

0.376
  pilB Glaesserella parasuis strain SC1401

39.528

95.225

0.376
  pilB Haemophilus influenzae Rd KW20

37.607

98.596

0.371

Multiple sequence alignment