Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MHI44_RS08540 Genome accession   NZ_CP150268
Coordinates   1617179..1618570 (-) Length   463 a.a.
NCBI ID   WP_070547860.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-1366     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1612179..1623570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI44_RS08505 (MHI44_08505) flgL 1612351..1613247 (-) 897 WP_015714797.1 flagellar hook-associated protein FlgL -
  MHI44_RS08510 (MHI44_08510) flgK 1613258..1614781 (-) 1524 WP_003228001.1 flagellar hook-associated protein FlgK -
  MHI44_RS08515 (MHI44_08515) flgN 1614800..1615282 (-) 483 WP_014478127.1 flagellar protein FlgN -
  MHI44_RS08520 (MHI44_08520) flgM 1615298..1615564 (-) 267 WP_014478128.1 flagellar biosynthesis anti-sigma factor FlgM -
  MHI44_RS08525 (MHI44_08525) - 1615645..1616064 (-) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  MHI44_RS08530 (MHI44_08530) comFC 1616137..1616859 (-) 723 WP_339203321.1 comF operon protein ComFC Machinery gene
  MHI44_RS08535 (MHI44_08535) comFB 1616823..1617119 (-) 297 WP_014478130.1 late competence protein ComFB -
  MHI44_RS08540 (MHI44_08540) comFA 1617179..1618570 (-) 1392 WP_070547860.1 ATP-dependent helicase ComFA Machinery gene
  MHI44_RS08545 (MHI44_08545) - 1618676..1619521 (-) 846 WP_003244125.1 DegV family protein -
  MHI44_RS08550 (MHI44_08550) degU 1619619..1620308 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  MHI44_RS08555 (MHI44_08555) degS 1620391..1621548 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  MHI44_RS08560 (MHI44_08560) - 1621765..1622418 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52557.52 Da        Isoelectric Point: 9.7976

>NTDB_id=968814 MHI44_RS08540 WP_070547860.1 1617179..1618570(-) (comFA) [Bacillus sp. FSL K6-1366]
MNVPVEKNSSFSRELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRGYRCNRCGQTDQRYYSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENEPNWQSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGAEISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=968814 MHI44_RS08540 WP_070547860.1 1617179..1618570(-) (comFA) [Bacillus sp. FSL K6-1366]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCTAGAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGGGATATAGATGTAATAGGTGCGGCCAAACTGATCAGCGGTATTATTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGGAAGAGGAAAATGA
ACCAAACTGGCAGTCAATTAAACTGACATGGGACGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGGCT
CAAGGCTGCCTTTCAGGGTGCTGAAATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGGGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

97.84

100

0.978

Multiple sequence alignment