Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MHI44_RS03420 Genome accession   NZ_CP150268
Coordinates   637100..637717 (-) Length   205 a.a.
NCBI ID   WP_029317886.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-1366     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 632100..642717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI44_RS03390 (MHI44_03390) rpsT 632402..632668 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  MHI44_RS03395 (MHI44_03395) holA 632683..633726 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  MHI44_RS03400 (MHI44_03400) - 633766..633915 (-) 150 WP_003229985.1 hypothetical protein -
  MHI44_RS03405 (MHI44_03405) - 633956..634090 (+) 135 WP_003229983.1 YqzM family protein -
  MHI44_RS03410 (MHI44_03410) comEC 634130..636460 (-) 2331 WP_339202871.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  MHI44_RS03415 (MHI44_03415) - 636464..637033 (-) 570 WP_003229978.1 ComE operon protein 2 -
  MHI44_RS03420 (MHI44_03420) comEA 637100..637717 (-) 618 WP_029317886.1 competence protein ComEA Machinery gene
  MHI44_RS03425 (MHI44_03425) comER 637801..638622 (+) 822 WP_014480313.1 late competence protein ComER -
  MHI44_RS03430 (MHI44_03430) - 638688..639431 (-) 744 WP_029317885.1 class I SAM-dependent methyltransferase -
  MHI44_RS03435 (MHI44_03435) rsfS 639428..639784 (-) 357 WP_014480315.1 ribosome silencing factor -
  MHI44_RS03440 (MHI44_03440) yqeK 639802..640362 (-) 561 WP_029317884.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MHI44_RS03445 (MHI44_03445) - 640352..640921 (-) 570 WP_339202875.1 nicotinate-nucleotide adenylyltransferase -
  MHI44_RS03450 (MHI44_03450) yhbY 640933..641223 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  MHI44_RS03455 (MHI44_03455) aroE 641217..642059 (-) 843 WP_029317883.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21722.44 Da        Isoelectric Point: 4.7220

>NTDB_id=968798 MHI44_RS03420 WP_029317886.1 637100..637717(-) (comEA) [Bacillus sp. FSL K6-1366]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGAVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFPTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=968798 MHI44_RS03420 WP_029317886.1 637100..637717(-) (comEA) [Bacillus sp. FSL K6-1366]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGGCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCCAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.024

100

0.99
  comEA Staphylococcus aureus MW2

36.364

100

0.39
  comEA Staphylococcus aureus N315

35.455

100

0.38

Multiple sequence alignment