Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MKY56_RS13120 Genome accession   NZ_CP150257
Coordinates   2520905..2521522 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus sp. FSL M8-0054     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2515905..2526522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY56_RS13090 (MKY56_13090) rpsT 2516207..2516473 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  MKY56_RS13095 (MKY56_13095) holA 2516488..2517531 (-) 1044 WP_339248831.1 DNA polymerase III subunit delta -
  MKY56_RS13100 (MKY56_13100) - 2517571..2517720 (-) 150 WP_120363468.1 hypothetical protein -
  MKY56_RS13105 (MKY56_13105) - 2517761..2517895 (+) 135 WP_003229983.1 YqzM family protein -
  MKY56_RS13110 (MKY56_13110) comEC 2517935..2520265 (-) 2331 WP_339248832.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  MKY56_RS13115 (MKY56_13115) - 2520269..2520838 (-) 570 WP_046381196.1 ComE operon protein 2 -
  MKY56_RS13120 (MKY56_13120) comEA 2520905..2521522 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  MKY56_RS13125 (MKY56_13125) comER 2521606..2522427 (+) 822 WP_014480313.1 late competence protein ComER -
  MKY56_RS13130 (MKY56_13130) - 2522494..2523237 (-) 744 WP_339248833.1 methyltransferase domain-containing protein -
  MKY56_RS13135 (MKY56_13135) rsfS 2523234..2523590 (-) 357 WP_014480315.1 ribosome silencing factor -
  MKY56_RS13140 (MKY56_13140) yqeK 2523608..2524168 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MKY56_RS13145 (MKY56_13145) - 2524158..2524727 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  MKY56_RS13150 (MKY56_13150) yhbY 2524739..2525029 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  MKY56_RS13155 (MKY56_13155) aroE 2525023..2525865 (-) 843 WP_038429328.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=968241 MKY56_RS13120 WP_004398514.1 2520905..2521522(-) (comEA) [Bacillus sp. FSL M8-0054]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=968241 MKY56_RS13120 WP_004398514.1 2520905..2521522(-) (comEA) [Bacillus sp. FSL M8-0054]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment