Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MKX71_RS12765 Genome accession   NZ_CP150256
Coordinates   2498466..2499083 (-) Length   205 a.a.
NCBI ID   WP_144483108.1    Uniprot ID   -
Organism   Bacillus sp. FSL M8-0071     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2493466..2504083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKX71_RS12735 (MKX71_12735) rpsT 2493768..2494034 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  MKX71_RS12740 (MKX71_12740) holA 2494049..2495092 (-) 1044 WP_015714286.1 DNA polymerase III subunit delta -
  MKX71_RS12745 (MKX71_12745) - 2495132..2495281 (-) 150 WP_003229985.1 hypothetical protein -
  MKX71_RS12750 (MKX71_12750) - 2495322..2495456 (+) 135 WP_003229983.1 YqzM family protein -
  MKX71_RS12755 (MKX71_12755) comEC 2495496..2497826 (-) 2331 WP_339231180.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  MKX71_RS12760 (MKX71_12760) - 2497830..2498399 (-) 570 WP_003229978.1 ComE operon protein 2 -
  MKX71_RS12765 (MKX71_12765) comEA 2498466..2499083 (-) 618 WP_144483108.1 competence protein ComEA Machinery gene
  MKX71_RS12770 (MKX71_12770) comER 2499167..2499988 (+) 822 WP_087961327.1 late competence protein ComER -
  MKX71_RS12775 (MKX71_12775) - 2500055..2500798 (-) 744 WP_015714290.1 class I SAM-dependent methyltransferase -
  MKX71_RS12780 (MKX71_12780) rsfS 2500795..2501151 (-) 357 WP_014477391.1 ribosome silencing factor -
  MKX71_RS12785 (MKX71_12785) yqeK 2501169..2501729 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MKX71_RS12790 (MKX71_12790) - 2501719..2502288 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  MKX71_RS12795 (MKX71_12795) yhbY 2502300..2502590 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  MKX71_RS12800 (MKX71_12800) aroE 2502584..2503426 (-) 843 WP_144483109.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21740.46 Da        Isoelectric Point: 4.8354

>NTDB_id=968162 MKX71_RS12765 WP_144483108.1 2498466..2499083(-) (comEA) [Bacillus sp. FSL M8-0071]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVRHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=968162 MKX71_RS12765 WP_144483108.1 2498466..2499083(-) (comEA) [Bacillus sp. FSL M8-0071]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCGGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.537

100

0.985
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment