Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MKY17_RS18690 Genome accession   NZ_CP150254
Coordinates   3895174..3896250 (-) Length   358 a.a.
NCBI ID   WP_098370095.1    Uniprot ID   -
Organism   Peribacillus sp. FSL P2-0133     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3890174..3901250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY17_RS18650 (MKY17_18650) - 3891034..3891834 (+) 801 WP_098369822.1 YqhG family protein -
  MKY17_RS18655 (MKY17_18655) - 3891882..3892067 (-) 186 WP_063233067.1 YqzE family protein -
  MKY17_RS18660 (MKY17_18660) comGG 3892185..3892571 (-) 387 WP_179890981.1 competence type IV pilus minor pilin ComGG -
  MKY17_RS18665 (MKY17_18665) comGF 3892564..3893043 (-) 480 WP_098369820.1 competence type IV pilus minor pilin ComGF -
  MKY17_RS18670 (MKY17_18670) - 3892991..3893329 (-) 339 WP_098369819.1 hypothetical protein -
  MKY17_RS18675 (MKY17_18675) comGD 3893316..3893759 (-) 444 WP_098369818.1 competence type IV pilus minor pilin ComGD -
  MKY17_RS18680 (MKY17_18680) comGC 3893767..3894078 (-) 312 WP_063233178.1 competence type IV pilus major pilin ComGC -
  MKY17_RS18685 (MKY17_18685) comGB 3894156..3895190 (-) 1035 WP_179890980.1 competence type IV pilus assembly protein ComGB -
  MKY17_RS18690 (MKY17_18690) comGA 3895174..3896250 (-) 1077 WP_098370095.1 competence type IV pilus ATPase ComGA Machinery gene
  MKY17_RS18695 (MKY17_18695) - 3896354..3896734 (-) 381 WP_098369816.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MKY17_RS18700 (MKY17_18700) - 3896947..3897648 (+) 702 WP_098369815.1 helix-turn-helix domain-containing protein -
  MKY17_RS18705 (MKY17_18705) - 3897734..3897976 (+) 243 WP_076365317.1 DUF2626 domain-containing protein -
  MKY17_RS18710 (MKY17_18710) - 3898029..3899129 (-) 1101 WP_179890979.1 SAM-dependent methyltransferase -
  MKY17_RS18715 (MKY17_18715) - 3899527..3900153 (-) 627 WP_098369813.1 MBL fold metallo-hydrolase -
  MKY17_RS18720 (MKY17_18720) - 3900386..3900559 (+) 174 WP_034308396.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39772.38 Da        Isoelectric Point: 8.4662

>NTDB_id=968022 MKY17_RS18690 WP_098370095.1 3895174..3896250(-) (comGA) [Peribacillus sp. FSL P2-0133]
MISIEKTAEKILTRAVQESASDIHIFFRKEGPLIQFRIDNKLVPKETLSFFEAERLIAHLKFLASMDIGEKRRPQSGAIT
INLANQVVGLRLSTLPTAHLESLVIRLIPQQNILPLEQLSLFPSTVQKLIALLKHSHGMLIFTGPTGSGKTTTLYSLLHH
AHEMINRNIITLEDPIENVSEKVLQVQINEKAGVTYSVGLKAVLRHDPDVIMVGEVRDAETAKIAVRAALTGHLILTTMH
TRDAQGAISRLLEFGVSLLEVEQSLIGVTAQRLVELRCLPCKGDCDLACKMTARNKRASVYELLYGKSLAEVLRIMGDEK
GKATVSYRQLKDEIGKAVAMGYVDSGEYERLVYDETKK

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=968022 MKY17_RS18690 WP_098370095.1 3895174..3896250(-) (comGA) [Peribacillus sp. FSL P2-0133]
TTGATATCGATTGAAAAGACCGCAGAAAAAATACTGACCCGTGCTGTACAGGAATCGGCATCGGATATCCACATTTTTTT
TCGCAAGGAGGGACCTCTCATCCAATTCAGGATAGACAATAAGCTTGTTCCAAAGGAAACATTATCATTCTTTGAAGCAG
AGAGGCTGATCGCTCATTTGAAGTTCCTTGCCTCGATGGATATAGGTGAGAAAAGGAGGCCCCAGAGTGGTGCCATCACC
ATCAATTTGGCCAACCAGGTGGTCGGACTCCGCCTTTCCACTTTACCCACTGCCCATCTCGAAAGTTTGGTCATCCGCTT
AATACCCCAACAGAATATCCTTCCTCTAGAACAGTTATCCTTATTTCCAAGCACCGTTCAAAAATTGATTGCTCTCCTGA
AGCATTCCCATGGCATGCTCATATTTACCGGCCCGACTGGCAGTGGAAAAACCACGACACTATATTCCCTGCTTCACCAT
GCCCATGAGATGATCAATCGAAATATTATTACACTTGAAGATCCCATTGAAAATGTATCCGAAAAGGTATTGCAAGTCCA
AATCAATGAAAAGGCGGGCGTTACGTATTCTGTTGGTCTAAAAGCTGTTCTGAGGCATGACCCTGACGTGATCATGGTTG
GGGAAGTCAGAGATGCAGAAACCGCCAAAATCGCAGTGCGTGCCGCATTGACCGGTCATTTGATACTTACAACCATGCAT
ACCAGGGACGCCCAGGGCGCCATCTCCAGGTTACTGGAATTTGGTGTCAGCCTGCTTGAGGTTGAACAGAGTTTGATTGG
CGTGACAGCACAGCGGCTGGTTGAATTGCGATGTCTTCCATGTAAAGGGGACTGTGATTTAGCTTGCAAAATGACTGCCA
GGAATAAAAGGGCAAGTGTATATGAATTGCTATATGGAAAAAGCCTGGCTGAGGTTCTCCGGATAATGGGAGATGAAAAA
GGAAAGGCAACGGTCAGCTACCGCCAATTGAAGGATGAAATCGGAAAAGCGGTTGCGATGGGATATGTGGATTCCGGGGA
ATATGAACGGCTGGTATACGATGAAACCAAAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.022

99.721

0.559
  pilB Haemophilus influenzae 86-028NP

39.031

98.045

0.383
  pilB Haemophilus influenzae Rd KW20

37.607

98.045

0.369

Multiple sequence alignment