Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OO774_RS01540 Genome accession   NZ_AP026763
Coordinates   325003..326016 (+) Length   337 a.a.
NCBI ID   WP_264904118.1    Uniprot ID   -
Organism   Vibrio sp. STUT-A11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 320003..331016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO774_RS01525 (VspSTUT11_02510) - 320426..321154 (-) 729 WP_264904115.1 glutathione peroxidase -
  OO774_RS01530 (VspSTUT11_02520) oxyR 321313..322221 (+) 909 WP_264904116.1 DNA-binding transcriptional regulator OxyR -
  OO774_RS01535 (VspSTUT11_02530) - 322292..324859 (-) 2568 WP_264904117.1 PBP1A family penicillin-binding protein -
  OO774_RS01540 (VspSTUT11_02540) pilM 325003..326016 (+) 1014 WP_264904118.1 type IV pilus assembly protein PilM Machinery gene
  OO774_RS01545 (VspSTUT11_02550) pilN 326000..326575 (+) 576 WP_264904119.1 PilN domain-containing protein Machinery gene
  OO774_RS01550 (VspSTUT11_02560) pilO 326568..327161 (+) 594 WP_264904120.1 type 4a pilus biogenesis protein PilO Machinery gene
  OO774_RS01555 (VspSTUT11_02570) pilP 327151..327666 (+) 516 WP_264904121.1 pilus assembly protein PilP Machinery gene
  OO774_RS01560 (VspSTUT11_02580) pilQ 327695..329425 (+) 1731 WP_264904122.1 type IV pilus secretin PilQ family protein Machinery gene
  OO774_RS01565 (VspSTUT11_02590) aroK 329614..330132 (+) 519 WP_014233147.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 337 a.a.        Molecular weight: 37322.49 Da        Isoelectric Point: 6.7239

>NTDB_id=96790 OO774_RS01540 WP_264904118.1 325003..326016(+) (pilM) [Vibrio sp. STUT-A11]
MDKRIVTGIDIGHNSLKAVVLKPNGDQFALLGYKEILLKEGIVAENNTINHQEIVNTLKQLKKQLPFGAKKVAISVPDNA
VISKKLHIEQNTEESEIEFAVVQAFSHQSPFPVDELSLDFVRLGTEESLRGPHHYQVFATRKDVVESRVEAIHKSGLKPV
LIDVHSQSLGQIWKLAAERFPDKSKYGLLDVGCQSSSFTMFTEQGELFHKEFAGGTGSIQASTTENPLAEEQAKTNQFNR
QVVDRVKRQIQLYTSINGSQNIKGLWLSGEGSTTPMLAEELSHQLALECELLNPLGLFEMNVAIRKRRAADWPHFSTAAG
LAVRGIQWLGGDCAISN

Nucleotide


Download         Length: 1014 bp        

>NTDB_id=96790 OO774_RS01540 WP_264904118.1 325003..326016(+) (pilM) [Vibrio sp. STUT-A11]
ATGGATAAGCGAATCGTTACAGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTCGTACTTAAACCCAATGGCGATCA
ATTCGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAATACCCTTAAACAGTTAAAAAAACAGCTGCCATTTGGCGCGAAAAAAGTAGCAATTTCAGTCCCTGATAATGCG
GTTATCAGCAAAAAACTACACATTGAACAAAATACCGAAGAGAGCGAAATAGAGTTTGCGGTAGTGCAGGCTTTTTCTCA
TCAATCTCCTTTCCCAGTGGACGAGCTGAGCCTAGATTTTGTCCGCCTTGGTACCGAAGAAAGTTTACGAGGGCCTCATC
ACTACCAAGTATTTGCAACGCGTAAAGATGTCGTAGAAAGCCGGGTTGAGGCAATACACAAGTCTGGCCTTAAACCTGTC
CTGATTGATGTTCATTCACAAAGCCTCGGACAGATATGGAAACTGGCGGCAGAGCGCTTCCCGGACAAAAGCAAGTACGG
TTTACTCGATGTAGGTTGTCAGTCGAGTTCATTCACCATGTTCACCGAGCAAGGTGAGCTGTTCCATAAAGAGTTTGCCG
GTGGAACGGGTTCTATTCAGGCTTCGACTACGGAAAACCCTCTTGCCGAAGAACAAGCAAAAACGAATCAGTTCAACCGT
CAGGTGGTCGATCGCGTAAAACGCCAGATACAGCTTTATACATCCATTAACGGCTCACAAAACATTAAAGGTCTTTGGTT
ATCCGGCGAAGGGTCAACAACGCCAATGTTGGCTGAAGAGCTTTCACATCAATTAGCGCTTGAATGCGAGCTACTGAACC
CGTTGGGCTTATTTGAAATGAACGTTGCCATACGCAAGCGCCGAGCGGCGGACTGGCCGCATTTTTCAACCGCCGCGGGT
TTGGCAGTACGAGGTATTCAATGGTTAGGAGGTGATTGTGCTATATCGAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-325)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

76.923

100

0.772
  pilM Vibrio cholerae strain A1552

49.704

100

0.499

Multiple sequence alignment