Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   NYE20_RS14015 Genome accession   NZ_CP150252
Coordinates   2637727..2638344 (-) Length   205 a.a.
NCBI ID   WP_141949657.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0416     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2632727..2643344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYE20_RS13985 (NYE20_13985) rpsT 2633029..2633295 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  NYE20_RS13990 (NYE20_13990) holA 2633310..2634353 (-) 1044 WP_161621676.1 DNA polymerase III subunit delta -
  NYE20_RS13995 (NYE20_13995) - 2634393..2634542 (-) 150 WP_003229985.1 hypothetical protein -
  NYE20_RS14000 (NYE20_14000) - 2634583..2634717 (+) 135 WP_003229983.1 YqzM family protein -
  NYE20_RS14005 (NYE20_14005) comEC 2634757..2637087 (-) 2331 WP_161621677.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NYE20_RS14010 (NYE20_14010) - 2637091..2637660 (-) 570 WP_003229978.1 ComE operon protein 2 -
  NYE20_RS14015 (NYE20_14015) comEA 2637727..2638344 (-) 618 WP_141949657.1 competence protein ComEA Machinery gene
  NYE20_RS14020 (NYE20_14020) comER 2638428..2639249 (+) 822 WP_014477389.1 late competence protein ComER -
  NYE20_RS14025 (NYE20_14025) - 2639315..2640058 (-) 744 WP_021480065.1 class I SAM-dependent methyltransferase -
  NYE20_RS14030 (NYE20_14030) rsfS 2640055..2640411 (-) 357 WP_014477391.1 ribosome silencing factor -
  NYE20_RS14035 (NYE20_14035) yqeK 2640429..2640989 (-) 561 WP_161621678.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NYE20_RS14040 (NYE20_14040) - 2640979..2641548 (-) 570 WP_021480066.1 nicotinate-nucleotide adenylyltransferase -
  NYE20_RS14045 (NYE20_14045) yhbY 2641560..2641850 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  NYE20_RS14050 (NYE20_14050) aroE 2641844..2642686 (-) 843 WP_014480317.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21811.53 Da        Isoelectric Point: 4.7416

>NTDB_id=967893 NYE20_RS14015 WP_141949657.1 2637727..2638344(-) (comEA) [Bacillus sp. FSL R5-0416]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGDGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=967893 NYE20_RS14015 WP_141949657.1 2637727..2638344(-) (comEA) [Bacillus sp. FSL R5-0416]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTACAGCAAGGCGACGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.073

100

0.971
  comEA Staphylococcus aureus MW2

35.455

100

0.38
  comEA Staphylococcus aureus N315

35.455

100

0.38

Multiple sequence alignment