Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   NYE20_RS13545 Genome accession   NZ_CP150252
Coordinates   2554761..2555144 (-) Length   127 a.a.
NCBI ID   WP_032726158.1    Uniprot ID   A0AAX3RJE0
Organism   Bacillus sp. FSL R5-0416     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2549761..2560144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYE20_RS13505 (NYE20_13505) sinI 2550695..2550868 (+) 174 WP_014477323.1 anti-repressor SinI family protein Regulator
  NYE20_RS13510 (NYE20_13510) sinR 2550902..2551237 (+) 336 WP_003226345.1 transcriptional regulator SinR Regulator
  NYE20_RS13515 (NYE20_13515) tasA 2551329..2552114 (-) 786 WP_014477324.1 biofilm matrix protein TasA -
  NYE20_RS13520 (NYE20_13520) - 2552179..2552751 (-) 573 WP_003246088.1 signal peptidase I -
  NYE20_RS13525 (NYE20_13525) tapA 2552735..2553496 (-) 762 WP_032726156.1 amyloid fiber anchoring/assembly protein TapA -
  NYE20_RS13530 (NYE20_13530) - 2553767..2554093 (+) 327 WP_038829733.1 YqzG/YhdC family protein -
  NYE20_RS13535 (NYE20_13535) - 2554135..2554314 (-) 180 WP_014480252.1 YqzE family protein -
  NYE20_RS13540 (NYE20_13540) comGG 2554386..2554760 (-) 375 WP_032726157.1 ComG operon protein ComGG Machinery gene
  NYE20_RS13545 (NYE20_13545) comGF 2554761..2555144 (-) 384 WP_032726158.1 ComG operon protein ComGF Machinery gene
  NYE20_RS13550 (NYE20_13550) comGE 2555170..2555517 (-) 348 WP_063335293.1 ComG operon protein 5 Machinery gene
  NYE20_RS13555 (NYE20_13555) comGD 2555501..2555932 (-) 432 WP_032726159.1 competence type IV pilus minor pilin ComGD Machinery gene
  NYE20_RS13560 (NYE20_13560) comGC 2555922..2556218 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  NYE20_RS13565 (NYE20_13565) comGB 2556232..2557269 (-) 1038 WP_086344083.1 comG operon protein ComGB Machinery gene
  NYE20_RS13570 (NYE20_13570) comGA 2557256..2558326 (-) 1071 WP_032726161.1 competence protein ComGA Machinery gene
  NYE20_RS13575 (NYE20_13575) - 2558538..2558735 (-) 198 WP_032726162.1 hypothetical protein -
  NYE20_RS13580 (NYE20_13580) corA 2558737..2559690 (-) 954 WP_161621668.1 magnesium transporter CorA -

Sequence

Protein


Download         Length: 127 a.a.        Molecular weight: 14315.39 Da        Isoelectric Point: 5.8929

>NTDB_id=967884 NYE20_RS13545 WP_032726158.1 2554761..2555144(-) (comGF) [Bacillus sp. FSL R5-0416]
MLISGSLAAIFHLFLSRQQEHDGFTQQEWMISIEQMMNECKESQAVKTAEHGSVLICTNLSGQDIRFDIYHSMIRKRVDG
KGHVPILDHITAMKADIENGVVLLKIESEDQKVYQTAFPVYSYLGGG

Nucleotide


Download         Length: 384 bp        

>NTDB_id=967884 NYE20_RS13545 WP_032726158.1 2554761..2555144(-) (comGF) [Bacillus sp. FSL R5-0416]
TTGCTCATATCAGGATCGTTAGCTGCGATTTTCCATCTGTTTTTATCTCGACAGCAGGAACATGACGGTTTCACACAGCA
AGAATGGATGATTTCGATAGAACAGATGATGAATGAATGCAAGGAATCACAGGCAGTTAAGACAGCCGAGCATGGGAGCG
TGTTAATCTGCACCAATCTTTCCGGACAAGACATCCGTTTTGACATTTATCATTCAATGATAAGAAAAAGAGTGGATGGC
AAAGGGCATGTTCCGATTTTAGATCATATTACTGCCATGAAAGCTGATATTGAAAATGGTGTTGTTTTGCTGAAAATTGA
GAGTGAGGACCAAAAAGTGTATCAAACTGCTTTTCCAGTCTATTCATATTTAGGAGGGGGGTGA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(16-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Bacillus subtilis subsp. subtilis str. 168

99.213

100

0.992

Multiple sequence alignment