Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   NYE53_RS12770 Genome accession   NZ_CP150251
Coordinates   2505428..2506045 (-) Length   205 a.a.
NCBI ID   WP_339245007.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0523     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2500428..2511045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYE53_RS12740 (NYE53_12740) rpsT 2500730..2500996 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  NYE53_RS12745 (NYE53_12745) holA 2501011..2502054 (-) 1044 WP_339245003.1 DNA polymerase III subunit delta -
  NYE53_RS12750 (NYE53_12750) - 2502094..2502243 (-) 150 WP_003229985.1 hypothetical protein -
  NYE53_RS12755 (NYE53_12755) - 2502284..2502418 (+) 135 WP_003229983.1 YqzM family protein -
  NYE53_RS12760 (NYE53_12760) comEC 2502458..2504788 (-) 2331 WP_339245005.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NYE53_RS12765 (NYE53_12765) - 2504792..2505361 (-) 570 WP_003229978.1 ComE operon protein 2 -
  NYE53_RS12770 (NYE53_12770) comEA 2505428..2506045 (-) 618 WP_339245007.1 competence protein ComEA Machinery gene
  NYE53_RS12775 (NYE53_12775) comER 2506129..2506950 (+) 822 WP_014477389.1 late competence protein ComER -
  NYE53_RS12780 (NYE53_12780) - 2507016..2507759 (-) 744 WP_339245010.1 class I SAM-dependent methyltransferase -
  NYE53_RS12785 (NYE53_12785) rsfS 2507756..2508112 (-) 357 WP_041333759.1 ribosome silencing factor -
  NYE53_RS12790 (NYE53_12790) yqeK 2508130..2508690 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NYE53_RS12795 (NYE53_12795) - 2508680..2509249 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  NYE53_RS12800 (NYE53_12800) yhbY 2509261..2509551 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  NYE53_RS12805 (NYE53_12805) aroE 2509545..2510387 (-) 843 WP_339245013.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21811.58 Da        Isoelectric Point: 4.8354

>NTDB_id=967813 NYE53_RS12770 WP_339245007.1 2505428..2506045(-) (comEA) [Bacillus sp. FSL R5-0523]
MNWLNQHKKAIILAASAVVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREEKGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=967813 NYE53_RS12770 WP_339245007.1 2505428..2506045(-) (comEA) [Bacillus sp. FSL R5-0523]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATCATTTTGGCGGCTTCTGCGGTTGTTTTCACAGCAATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAAAGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.024

100

0.99
  comEA Staphylococcus aureus MW2

37.727

100

0.405
  comEA Staphylococcus aureus N315

36.818

100

0.395

Multiple sequence alignment