Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   MKY61_RS14710 Genome accession   NZ_CP150247
Coordinates   2780305..2780598 (-) Length   97 a.a.
NCBI ID   WP_003183466.1    Uniprot ID   -
Organism   Bacillus sp. FSL M8-0315     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2775305..2785598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY61_RS14665 (MKY61_14665) - 2775681..2776475 (-) 795 WP_003183447.1 TasA family protein -
  MKY61_RS14670 (MKY61_14670) - 2776549..2777133 (-) 585 WP_003183449.1 signal peptidase I -
  MKY61_RS14675 (MKY61_14675) tapA 2777130..2777858 (-) 729 WP_011198112.1 amyloid fiber anchoring/assembly protein TapA -
  MKY61_RS14680 (MKY61_14680) - 2778168..2778455 (+) 288 WP_223307118.1 YqzG/YhdC family protein -
  MKY61_RS14685 (MKY61_14685) - 2778485..2778667 (-) 183 WP_003183456.1 YqzE family protein -
  MKY61_RS14690 (MKY61_14690) comGG 2778756..2779121 (-) 366 WP_003183459.1 competence type IV pilus minor pilin ComGG -
  MKY61_RS14695 (MKY61_14695) comGF 2779134..2779622 (-) 489 WP_011201694.1 competence type IV pilus minor pilin ComGF -
  MKY61_RS14700 (MKY61_14700) comGE 2779531..2779878 (-) 348 WP_026699160.1 competence type IV pilus minor pilin ComGE -
  MKY61_RS14705 (MKY61_14705) comGD 2779862..2780299 (-) 438 WP_071583679.1 competence type IV pilus minor pilin ComGD -
  MKY61_RS14710 (MKY61_14710) comGC 2780305..2780598 (-) 294 WP_003183466.1 competence type IV pilus major pilin ComGC Machinery gene
  MKY61_RS14715 (MKY61_14715) comGB 2780612..2781646 (-) 1035 WP_011198115.1 competence type IV pilus assembly protein ComGB Machinery gene
  MKY61_RS14720 (MKY61_14720) comGA 2781636..2782700 (-) 1065 WP_003183477.1 competence type IV pilus ATPase ComGA Machinery gene
  MKY61_RS14725 (MKY61_14725) - 2782857..2783696 (-) 840 WP_071583678.1 STAS domain-containing protein -
  MKY61_RS14735 (MKY61_14735) - 2783938..2784315 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MKY61_RS14740 (MKY61_14740) - 2784524..2784769 (+) 246 WP_003183483.1 DUF2626 domain-containing protein -
  MKY61_RS14745 (MKY61_14745) - 2784807..2785445 (-) 639 WP_071583677.1 MBL fold metallo-hydrolase -

Sequence


Protein


Download         Length: 97 a.a.        Molecular weight: 10725.62 Da        Isoelectric Point: 9.1200

>NTDB_id=967587 MKY61_RS14710 WP_003183466.1 2780305..2780598(-) (comGC) [Bacillus sp. FSL M8-0315]
MNEKGFTLIEMLVVMLVISILLLITIPNVTKHNQSIQKKGCEGLINMVQAQITAYQMDHDGKTPNRAELESEGYIKKNLK
CPNGKAIKISNGKAQAD

Nucleotide


Download         Length: 294 bp        

>NTDB_id=967587 MKY61_RS14710 WP_003183466.1 2780305..2780598(-) (comGC) [Bacillus sp. FSL M8-0315]
GTGAATGAAAAGGGATTTACACTGATAGAAATGTTAGTCGTCATGCTCGTTATCTCCATCCTGCTGTTGATCACCATTCC
GAATGTGACCAAGCATAACCAAAGCATCCAAAAGAAAGGATGCGAAGGATTAATCAATATGGTTCAAGCGCAGATCACGG
CCTATCAGATGGATCATGACGGGAAAACGCCGAACAGGGCGGAGCTTGAATCTGAAGGTTACATAAAAAAGAACTTAAAG
TGTCCGAATGGGAAAGCAATTAAAATTTCAAACGGCAAAGCACAGGCTGATTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

68.041

100

0.68

  comGC Staphylococcus aureus MW2

44.211

97.938

0.433

  comGC Staphylococcus aureus N315

44.211

97.938

0.433


Multiple sequence alignment