Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MKY61_RS14720 Genome accession   NZ_CP150247
Coordinates   2781636..2782700 (-) Length   354 a.a.
NCBI ID   WP_003183477.1    Uniprot ID   Q8VQ73
Organism   Bacillus sp. FSL M8-0315     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2776636..2787700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY61_RS14675 (MKY61_14675) tapA 2777130..2777858 (-) 729 WP_011198112.1 amyloid fiber anchoring/assembly protein TapA -
  MKY61_RS14680 (MKY61_14680) - 2778168..2778455 (+) 288 WP_223307118.1 YqzG/YhdC family protein -
  MKY61_RS14685 (MKY61_14685) - 2778485..2778667 (-) 183 WP_003183456.1 YqzE family protein -
  MKY61_RS14690 (MKY61_14690) comGG 2778756..2779121 (-) 366 WP_003183459.1 competence type IV pilus minor pilin ComGG -
  MKY61_RS14695 (MKY61_14695) comGF 2779134..2779622 (-) 489 WP_011201694.1 competence type IV pilus minor pilin ComGF -
  MKY61_RS14700 (MKY61_14700) comGE 2779531..2779878 (-) 348 WP_026699160.1 competence type IV pilus minor pilin ComGE -
  MKY61_RS14705 (MKY61_14705) comGD 2779862..2780299 (-) 438 WP_071583679.1 competence type IV pilus minor pilin ComGD -
  MKY61_RS14710 (MKY61_14710) comGC 2780305..2780598 (-) 294 WP_003183466.1 competence type IV pilus major pilin ComGC Machinery gene
  MKY61_RS14715 (MKY61_14715) comGB 2780612..2781646 (-) 1035 WP_011198115.1 competence type IV pilus assembly protein ComGB Machinery gene
  MKY61_RS14720 (MKY61_14720) comGA 2781636..2782700 (-) 1065 WP_003183477.1 competence type IV pilus ATPase ComGA Machinery gene
  MKY61_RS14725 (MKY61_14725) - 2782857..2783696 (-) 840 WP_071583678.1 STAS domain-containing protein -
  MKY61_RS14735 (MKY61_14735) - 2783938..2784315 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MKY61_RS14740 (MKY61_14740) - 2784524..2784769 (+) 246 WP_003183483.1 DUF2626 domain-containing protein -
  MKY61_RS14745 (MKY61_14745) - 2784807..2785445 (-) 639 WP_071583677.1 MBL fold metallo-hydrolase -
  MKY61_RS14750 (MKY61_14750) - 2785601..2785774 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  MKY61_RS14755 (MKY61_14755) - 2785835..2786149 (-) 315 WP_003183487.1 MTH1187 family thiamine-binding protein -
  MKY61_RS14760 (MKY61_14760) - 2786165..2787274 (-) 1110 WP_003183488.1 hypothetical protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39727.31 Da        Isoelectric Point: 9.1029

>NTDB_id=967589 MKY61_RS14720 WP_003183477.1 2781636..2782700(-) (comGA) [Bacillus sp. FSL M8-0315]
MYTIESLSGRLIDEAYRMKASDIHIVPGEKEAVVRFRIDDELFQKDGLTRNECSRLISHFKFLSSMDIGERRLPQSGALT
LYINREPVHLRMSTLPTIHDESLVIRLLPKMSSKPLTKLSLFPSATFKLLSFLKHSHGLMLFTGPTGSGKTTTLYSLIDY
AKRHFKRNIITLEDPVESRSENILQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAQIAVRAALTGHLVLSSMH
AKNAKGAIYRLLELGVDMTEIEQTLVAVSAQRLVDIVCPFCGDSCTLYCRLSRPVRRASVFELLYGKSLNLCIEEAKGRC
GGIKTDTLKMLIRKGIALGYLPSKTYERWIGHED

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=967589 MKY61_RS14720 WP_003183477.1 2781636..2782700(-) (comGA) [Bacillus sp. FSL M8-0315]
TTGTACACGATCGAATCATTAAGCGGAAGATTAATTGATGAGGCATATAGAATGAAGGCGTCAGACATTCATATTGTTCC
CGGTGAAAAAGAGGCGGTGGTACGCTTCAGGATTGATGACGAACTGTTTCAAAAAGACGGATTAACGAGAAATGAGTGCT
CTAGACTCATTTCTCACTTTAAATTTCTTTCTTCGATGGACATTGGCGAGAGACGGCTGCCGCAAAGCGGGGCTTTAACT
CTTTATATCAACCGTGAGCCCGTTCATTTAAGGATGTCCACTCTGCCCACCATCCATGATGAAAGCTTGGTCATCAGGCT
TCTTCCCAAGATGAGCAGCAAGCCGCTCACAAAGCTCTCATTGTTCCCGAGTGCAACATTCAAGCTGCTGTCTTTTCTGA
AGCATTCCCACGGATTAATGCTTTTCACCGGCCCGACAGGCTCGGGAAAAACGACAACACTCTATTCTTTAATTGACTAC
GCCAAGCGGCATTTTAAGCGAAATATCATTACGTTGGAAGACCCGGTCGAATCAAGAAGCGAAAACATTTTGCAAGTACA
AGTGAACGAAAAAGCGGGCATGACTTATTCTGCGGGGTTGAAGGCGGTGCTGCGCCACGATCCGGACATGATCATCCTAG
GGGAAATTCGCGATGCCGAAACAGCCCAAATCGCAGTCAGGGCAGCTTTGACGGGACACCTTGTTCTATCGAGCATGCAT
GCGAAAAATGCTAAAGGTGCGATATACAGGCTGCTCGAGCTCGGCGTTGATATGACAGAAATTGAACAAACATTGGTCGC
AGTCAGCGCCCAGCGTCTTGTCGATATCGTCTGTCCGTTTTGCGGCGACAGCTGCACTTTGTATTGCAGATTGTCGAGAC
CAGTTAGAAGAGCAAGCGTCTTTGAACTTTTGTACGGAAAAAGCCTTAATCTCTGCATCGAAGAAGCAAAAGGCAGATGC
GGTGGCATCAAAACAGACACCTTAAAAATGCTGATCCGCAAGGGAATAGCACTCGGATATCTGCCGTCGAAAACTTATGA
ACGCTGGATAGGCCATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VQ73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

66.949

100

0.669

Multiple sequence alignment