Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   NST56_RS14165 Genome accession   NZ_CP150195
Coordinates   2668343..2668960 (-) Length   205 a.a.
NCBI ID   WP_326203057.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0560     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2663343..2673960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST56_RS14135 (NST56_14135) rpsT 2663646..2663912 (+) 267 WP_010335003.1 30S ribosomal protein S20 -
  NST56_RS14140 (NST56_14140) holA 2663930..2664970 (-) 1041 WP_010335004.1 DNA polymerase III subunit delta -
  NST56_RS14145 (NST56_14145) - 2665010..2665159 (-) 150 WP_003229985.1 hypothetical protein -
  NST56_RS14150 (NST56_14150) - 2665200..2665334 (+) 135 WP_010335005.1 YqzM family protein -
  NST56_RS14155 (NST56_14155) comEC 2665373..2667703 (-) 2331 WP_339182235.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NST56_RS14160 (NST56_14160) - 2667707..2668276 (-) 570 WP_010335007.1 ComE operon protein 2 -
  NST56_RS14165 (NST56_14165) comEA 2668343..2668960 (-) 618 WP_326203057.1 helix-hairpin-helix domain-containing protein Machinery gene
  NST56_RS14170 (NST56_14170) comER 2669044..2669865 (+) 822 WP_339177269.1 late competence protein ComER -
  NST56_RS14175 (NST56_14175) - 2669930..2670673 (-) 744 WP_229146794.1 class I SAM-dependent methyltransferase -
  NST56_RS14180 (NST56_14180) rsfS 2670670..2671026 (-) 357 WP_010335011.1 ribosome silencing factor -
  NST56_RS14185 (NST56_14185) yqeK 2671044..2671604 (-) 561 WP_339177275.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NST56_RS14190 (NST56_14190) - 2671594..2672163 (-) 570 WP_339177278.1 nicotinate-nucleotide adenylyltransferase -
  NST56_RS14195 (NST56_14195) yhbY 2672174..2672464 (-) 291 WP_326195918.1 ribosome assembly RNA-binding protein YhbY -
  NST56_RS14200 (NST56_14200) aroE 2672464..2673300 (-) 837 WP_339177286.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 22109.05 Da        Isoelectric Point: 4.6750

>NTDB_id=965860 NST56_RS14165 WP_326203057.1 2668343..2668960(-) (comEA) [Bacillus sp. FSL R5-0560]
MNWLNQHKMIIMSAAFAAVLIAVLCLFFFIGHNKEPVKQVISSEKDNAEVKQEVTKDKAEENIVIDIKGAVKHPGVYEMH
AGDRVSQAIEKAGGIGEKADETQVNLAELLQDGTVVYIPSEGEEEPQQAAGGAVQSSTGKEALVNINTATLEDLQNISGV
GPSKAEAIIAYREENGRFQAIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=965860 NST56_RS14165 WP_326203057.1 2668343..2668960(-) (comEA) [Bacillus sp. FSL R5-0560]
TTGAATTGGTTGAATCAACATAAAATGATCATCATGTCAGCGGCCTTTGCTGCTGTTCTCATTGCGGTTCTTTGCCTCTT
TTTTTTCATCGGGCATAACAAAGAGCCTGTAAAACAAGTGATATCTAGTGAAAAGGATAATGCTGAGGTCAAGCAGGAAG
TGACAAAAGACAAAGCGGAAGAAAATATTGTAATTGACATAAAAGGTGCTGTTAAGCATCCCGGCGTTTATGAAATGCAT
GCAGGGGACAGAGTGTCACAGGCTATTGAGAAAGCAGGCGGCATCGGCGAAAAAGCAGATGAAACGCAGGTGAATTTGGC
GGAACTTTTGCAGGACGGTACAGTGGTGTATATACCGAGTGAGGGAGAAGAGGAGCCGCAGCAAGCAGCCGGAGGCGCTG
TACAAAGCAGTACGGGGAAAGAGGCCTTGGTCAATATCAATACAGCAACATTAGAGGATTTACAAAACATTTCAGGAGTA
GGGCCTTCAAAAGCCGAAGCGATTATTGCATATCGGGAAGAGAACGGGCGTTTTCAGGCAATTGAAGATATCACAAAGGT
TTCGGGAATTGGCGAAAAATCATTTGAAAAAATTAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-203)

SLBB domain (SLBB)

(64-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

74.757

100

0.751
  comEA Staphylococcus aureus MW2

38.182

100

0.41
  comEA Staphylococcus aureus N315

37.387

100

0.405

Multiple sequence alignment