Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   NSQ42_RS12955 Genome accession   NZ_CP150184
Coordinates   2502804..2503421 (-) Length   205 a.a.
NCBI ID   WP_088467267.1    Uniprot ID   -
Organism   Bacillus sp. FSL W8-0812     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2497804..2508421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ42_RS12925 (NSQ42_12925) rpsT 2498106..2498372 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  NSQ42_RS12930 (NSQ42_12930) holA 2498387..2499430 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  NSQ42_RS12935 (NSQ42_12935) - 2499470..2499619 (-) 150 WP_003229985.1 hypothetical protein -
  NSQ42_RS12940 (NSQ42_12940) - 2499660..2499794 (+) 135 WP_003229983.1 YqzM family protein -
  NSQ42_RS12945 (NSQ42_12945) comEC 2499834..2502164 (-) 2331 WP_339225583.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NSQ42_RS12950 (NSQ42_12950) - 2502168..2502737 (-) 570 WP_003229978.1 ComE operon protein 2 -
  NSQ42_RS12955 (NSQ42_12955) comEA 2502804..2503421 (-) 618 WP_088467267.1 competence protein ComEA Machinery gene
  NSQ42_RS12960 (NSQ42_12960) comER 2503505..2504326 (+) 822 WP_004398597.1 late competence protein ComER -
  NSQ42_RS12965 (NSQ42_12965) - 2504392..2505135 (-) 744 WP_015384134.1 class I SAM-dependent methyltransferase -
  NSQ42_RS12970 (NSQ42_12970) rsfS 2505132..2505488 (-) 357 WP_014477391.1 ribosome silencing factor -
  NSQ42_RS12975 (NSQ42_12975) yqeK 2505506..2506066 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NSQ42_RS12980 (NSQ42_12980) - 2506056..2506625 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  NSQ42_RS12985 (NSQ42_12985) yhbY 2506637..2506927 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  NSQ42_RS12990 (NSQ42_12990) aroE 2506921..2507763 (-) 843 WP_088467268.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21840.58 Da        Isoelectric Point: 4.8354

>NTDB_id=965374 NSQ42_RS12955 WP_088467267.1 2502804..2503421(-) (comEA) [Bacillus sp. FSL W8-0812]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESSETIVIDIKGAVRHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGTGGSVQSDGGKGPLVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=965374 NSQ42_RS12955 WP_088467267.1 2502804..2503421(-) (comEA) [Bacillus sp. FSL W8-0812]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGTGAAACAATTGTGATAGACATCAAAGGTGCTGTTCGGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTACCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGGCCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.049

100

0.98
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment