Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   NSU05_RS12825 Genome accession   NZ_CP150177
Coordinates   2509610..2510227 (-) Length   205 a.a.
NCBI ID   WP_038429326.1    Uniprot ID   -
Organism   Bacillus sp. EU52     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2504610..2515227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSU05_RS12795 (NSU05_12795) rpsT 2504912..2505178 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  NSU05_RS12800 (NSU05_12800) holA 2505193..2506236 (-) 1044 WP_038429324.1 DNA polymerase III subunit delta -
  NSU05_RS12805 (NSU05_12805) - 2506276..2506425 (-) 150 WP_003229985.1 hypothetical protein -
  NSU05_RS12810 (NSU05_12810) - 2506466..2506600 (+) 135 WP_003229983.1 YqzM family protein -
  NSU05_RS12815 (NSU05_12815) comEC 2506640..2508970 (-) 2331 WP_038429325.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NSU05_RS12820 (NSU05_12820) - 2508974..2509543 (-) 570 WP_003229978.1 ComE operon protein 2 -
  NSU05_RS12825 (NSU05_12825) comEA 2509610..2510227 (-) 618 WP_038429326.1 competence protein ComEA Machinery gene
  NSU05_RS12830 (NSU05_12830) comER 2510311..2511132 (+) 822 WP_004398597.1 late competence protein ComER -
  NSU05_RS12835 (NSU05_12835) - 2511198..2511941 (-) 744 WP_038429327.1 class I SAM-dependent methyltransferase -
  NSU05_RS12840 (NSU05_12840) rsfS 2511938..2512294 (-) 357 WP_014480315.1 ribosome silencing factor -
  NSU05_RS12845 (NSU05_12845) yqeK 2512312..2512872 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NSU05_RS12850 (NSU05_12850) - 2512862..2513431 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  NSU05_RS12855 (NSU05_12855) yhbY 2513443..2513733 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  NSU05_RS12860 (NSU05_12860) aroE 2513727..2514569 (-) 843 WP_038429328.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21770.48 Da        Isoelectric Point: 4.8354

>NTDB_id=965096 NSU05_RS12825 WP_038429326.1 2509610..2510227(-) (comEA) [Bacillus sp. EU52]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESSETIVIDIKGAVRHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=965096 NSU05_RS12825 WP_038429326.1 2509610..2510227(-) (comEA) [Bacillus sp. EU52]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTCTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCGGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.024

100

0.99
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment