Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NST31_RS14500 Genome accession   NZ_CP150169
Coordinates   3108114..3109244 (+) Length   376 a.a.
NCBI ID   WP_339161574.1    Uniprot ID   -
Organism   Siminovitchia sp. FSL W7-1587     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3103114..3114244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST31_RS14490 (NST31_14490) - 3105035..3106867 (-) 1833 WP_339161569.1 long-chain fatty acid--CoA ligase -
  NST31_RS14495 (NST31_14495) - 3107093..3107440 (-) 348 WP_339161571.1 SCP2 sterol-binding domain-containing protein -
  NST31_RS14500 (NST31_14500) comFA 3108114..3109244 (+) 1131 WP_339161574.1 DEAD/DEAH box helicase Machinery gene
  NST31_RS14505 (NST31_14505) - 3109237..3109944 (+) 708 WP_077111646.1 ComF family protein -
  NST31_RS14510 (NST31_14510) flgM 3110020..3110280 (+) 261 WP_339161579.1 flagellar biosynthesis anti-sigma factor FlgM -
  NST31_RS14515 (NST31_14515) - 3110299..3110778 (+) 480 WP_077111644.1 flagellar protein FlgN -
  NST31_RS14520 (NST31_14520) flgK 3110796..3112214 (+) 1419 WP_339161582.1 flagellar hook-associated protein FlgK -
  NST31_RS14525 (NST31_14525) flgL 3112228..3113079 (+) 852 WP_077111642.1 flagellar hook-associated protein FlgL -
  NST31_RS14530 (NST31_14530) - 3113356..3113943 (+) 588 WP_339161585.1 DUF6470 family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42050.81 Da        Isoelectric Point: 9.9021

>NTDB_id=964675 NST31_RS14500 WP_339161574.1 3108114..3109244(+) (comFA) [Siminovitchia sp. FSL W7-1587]
MMGRVASCTTLYRWSGPEPEWADAVALEWDGQLSKAQNAAAAAVLSAVKKEEELLVWAVCGAGKTEVLFSGIEAALQLGK
RTCIATPRTDVALELAPRLQKVFPSIAVAVLYGGSEDRHVFSPLSISTTHQLMRFERAFDVMIVDEVDAFPYSFDQSLQW
AVEKAAKKNAARIYLTATPSKRWQEECRYGKRNFIKIPARYHRKPLPAPVFVWCGNWRKSFEKSKLPRVVLKWVRERLQA
GKQALIFLPHIQLMENSLPLFKQLHSSIQAVHAEDPLREEKVNAMRREKIPILLTTTILERGVTFSNLDVAVIGAEDPIF
TEAALVQIAGRVGRSADFPGGNVTFFHHGKTKAMIQALVHINHMNKAARKAGLIDE

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=964675 NST31_RS14500 WP_339161574.1 3108114..3109244(+) (comFA) [Siminovitchia sp. FSL W7-1587]
ATGATGGGCAGGGTGGCTTCGTGCACAACCCTGTACCGCTGGTCCGGCCCGGAGCCCGAATGGGCGGATGCGGTGGCTCT
TGAGTGGGACGGACAATTATCCAAGGCGCAAAACGCCGCGGCGGCCGCAGTTTTAAGTGCTGTCAAGAAAGAGGAAGAAC
TGCTGGTATGGGCGGTATGCGGGGCGGGAAAAACAGAGGTCCTTTTTTCAGGGATTGAAGCAGCGCTCCAACTTGGCAAG
AGAACTTGCATTGCCACTCCGCGTACAGACGTCGCCTTGGAGCTTGCTCCACGATTGCAAAAAGTGTTTCCTTCGATAGC
TGTGGCTGTTTTGTACGGTGGGAGTGAAGACCGGCATGTATTTTCTCCTCTGAGTATATCTACTACCCACCAGCTCATGC
GTTTTGAACGCGCTTTTGATGTCATGATCGTCGATGAAGTGGATGCTTTCCCCTATTCTTTTGATCAAAGCTTGCAATGG
GCAGTTGAAAAAGCGGCGAAGAAAAATGCCGCTCGCATTTATTTAACGGCAACGCCAAGCAAGCGCTGGCAGGAAGAATG
CCGTTATGGAAAAAGAAACTTTATCAAGATTCCTGCCCGCTATCACCGCAAGCCACTCCCGGCTCCCGTATTTGTTTGGT
GTGGCAATTGGCGCAAATCTTTTGAAAAAAGCAAACTCCCCCGTGTTGTACTAAAGTGGGTCCGTGAACGGCTCCAAGCA
GGAAAACAGGCTCTCATTTTTCTCCCTCATATTCAACTCATGGAAAACTCCCTTCCATTATTTAAACAGCTTCATTCGTC
TATTCAGGCCGTACATGCAGAGGACCCATTAAGAGAAGAAAAAGTGAATGCGATGAGGAGAGAGAAAATCCCGATTCTTT
TAACGACAACGATTTTGGAAAGAGGAGTCACCTTTTCAAATTTGGATGTAGCGGTGATCGGTGCAGAAGATCCTATTTTC
ACTGAGGCGGCTCTCGTCCAAATTGCCGGACGTGTTGGCAGAAGCGCCGATTTTCCTGGCGGGAATGTCACTTTTTTTCA
TCATGGCAAGACGAAAGCGATGATCCAAGCCCTCGTTCATATTAATCATATGAATAAAGCAGCCCGAAAAGCGGGATTGA
TTGATGAATAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(32-180)

Helicase conserved C-terminal domain (Helicase_C)

(236-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.733

99.734

0.516
  comFA/cflA Streptococcus mitis SK321

39.73

98.404

0.391
  comFA/cflA Streptococcus mitis NCTC 12261

39.73

98.404

0.391
  comFA/cflA Streptococcus pneumoniae Rx1

38.649

98.404

0.38
  comFA/cflA Streptococcus pneumoniae D39

38.649

98.404

0.38
  comFA/cflA Streptococcus pneumoniae R6

38.649

98.404

0.38
  comFA Latilactobacillus sakei subsp. sakei 23K

40.688

92.819

0.378
  comFA/cflA Streptococcus pneumoniae TIGR4

38.378

98.404

0.378
  comFA Lactococcus lactis subsp. cremoris KW2

37.875

97.606

0.37

Multiple sequence alignment