Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   NSQ53_RS14010 Genome accession   NZ_CP150164
Coordinates   2662714..2663331 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus sp. PS11(2022) isolate PS11     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2657714..2668331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ53_RS13980 (NSQ53_13980) rpsT 2658016..2658282 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  NSQ53_RS13985 (NSQ53_13985) holA 2658297..2659340 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  NSQ53_RS13990 (NSQ53_13990) - 2659380..2659529 (-) 150 WP_003229985.1 hypothetical protein -
  NSQ53_RS13995 (NSQ53_13995) - 2659570..2659704 (+) 135 WP_003229983.1 YqzM family protein -
  NSQ53_RS14000 (NSQ53_14000) comEC 2659744..2662074 (-) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NSQ53_RS14005 (NSQ53_14005) - 2662078..2662647 (-) 570 WP_003229978.1 ComE operon protein 2 -
  NSQ53_RS14010 (NSQ53_14010) comEA 2662714..2663331 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  NSQ53_RS14015 (NSQ53_14015) comER 2663415..2664236 (+) 822 WP_004398597.1 late competence protein ComER -
  NSQ53_RS14020 (NSQ53_14020) - 2664302..2665045 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  NSQ53_RS14025 (NSQ53_14025) rsfS 2665042..2665398 (-) 357 WP_003229971.1 ribosome silencing factor -
  NSQ53_RS14030 (NSQ53_14030) yqeK 2665416..2665976 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NSQ53_RS14035 (NSQ53_14035) - 2665966..2666535 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  NSQ53_RS14040 (NSQ53_14040) yhbY 2666547..2666837 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  NSQ53_RS14045 (NSQ53_14045) aroE 2666831..2667673 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=964417 NSQ53_RS14010 WP_004398514.1 2662714..2663331(-) (comEA) [Bacillus sp. PS11(2022) isolate PS11]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=964417 NSQ53_RS14010 WP_004398514.1 2662714..2663331(-) (comEA) [Bacillus sp. PS11(2022) isolate PS11]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment