Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   NST79_RS13320 Genome accession   NZ_CP150159
Coordinates   2563096..2563713 (-) Length   205 a.a.
NCBI ID   WP_041850029.1    Uniprot ID   -
Organism   Bacillus sp. PS196     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2558096..2568713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST79_RS13290 (NST79_13290) rpsT 2558398..2558664 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  NST79_RS13295 (NST79_13295) holA 2558679..2559722 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  NST79_RS13300 (NST79_13300) - 2559762..2559911 (-) 150 WP_120363468.1 hypothetical protein -
  NST79_RS13305 (NST79_13305) - 2559952..2560086 (+) 135 WP_003229983.1 YqzM family protein -
  NST79_RS13310 (NST79_13310) comEC 2560126..2562456 (-) 2331 WP_041850028.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NST79_RS13315 (NST79_13315) - 2562457..2563029 (-) 573 WP_212060334.1 ComE operon protein 2 -
  NST79_RS13320 (NST79_13320) comEA 2563096..2563713 (-) 618 WP_041850029.1 competence protein ComEA Machinery gene
  NST79_RS13325 (NST79_13325) comER 2563797..2564618 (+) 822 WP_041850030.1 late competence protein ComER -
  NST79_RS13330 (NST79_13330) - 2564684..2565427 (-) 744 WP_042976537.1 class I SAM-dependent methyltransferase -
  NST79_RS13335 (NST79_13335) rsfS 2565424..2565780 (-) 357 WP_014480315.1 ribosome silencing factor -
  NST79_RS13340 (NST79_13340) yqeK 2565798..2566358 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NST79_RS13345 (NST79_13345) - 2566348..2566917 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  NST79_RS13350 (NST79_13350) yhbY 2566929..2567219 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  NST79_RS13355 (NST79_13355) aroE 2567213..2568055 (-) 843 WP_212060328.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21821.62 Da        Isoelectric Point: 4.7220

>NTDB_id=964001 NST79_RS13320 WP_041850029.1 2563096..2563713(-) (comEA) [Bacillus sp. PS196]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEVQVNLAEILQDGIVVYIPKKGEETAVQQGGGVSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=964001 NST79_RS13320 WP_041850029.1 2563096..2563713(-) (comEA) [Bacillus sp. PS196]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCAATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGTGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGATAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGTGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATATCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-117)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.049

100

0.98
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

35.455

100

0.38