Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   NSQ83_RS12965 Genome accession   NZ_CP150157
Coordinates   2519257..2519874 (-) Length   205 a.a.
NCBI ID   WP_198878617.1    Uniprot ID   -
Organism   Bacillus sp. PS217     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2514257..2524874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ83_RS12935 (NSQ83_12935) rpsT 2514559..2514825 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  NSQ83_RS12940 (NSQ83_12940) holA 2514840..2515883 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  NSQ83_RS12945 (NSQ83_12945) - 2515923..2516072 (-) 150 WP_003229985.1 hypothetical protein -
  NSQ83_RS12950 (NSQ83_12950) - 2516113..2516247 (+) 135 WP_003229983.1 YqzM family protein -
  NSQ83_RS12955 (NSQ83_12955) comEC 2516287..2518617 (-) 2331 WP_198878618.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NSQ83_RS12960 (NSQ83_12960) - 2518621..2519190 (-) 570 WP_003229978.1 ComE operon protein 2 -
  NSQ83_RS12965 (NSQ83_12965) comEA 2519257..2519874 (-) 618 WP_198878617.1 competence protein ComEA Machinery gene
  NSQ83_RS12970 (NSQ83_12970) comER 2519958..2520779 (+) 822 WP_014480313.1 late competence protein ComER -
  NSQ83_RS12975 (NSQ83_12975) - 2520845..2521588 (-) 744 WP_198878616.1 class I SAM-dependent methyltransferase -
  NSQ83_RS12980 (NSQ83_12980) rsfS 2521585..2521941 (-) 357 WP_014480315.1 ribosome silencing factor -
  NSQ83_RS12985 (NSQ83_12985) yqeK 2521959..2522519 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NSQ83_RS12990 (NSQ83_12990) - 2522509..2523078 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  NSQ83_RS12995 (NSQ83_12995) yhbY 2523090..2523380 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  NSQ83_RS13000 (NSQ83_13000) aroE 2523374..2524216 (-) 843 WP_076458133.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21839.63 Da        Isoelectric Point: 4.9739

>NTDB_id=963839 NSQ83_RS12965 WP_198878617.1 2519257..2519874(-) (comEA) [Bacillus sp. PS217]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESSETIVIDIKGAVKHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGTGGSVQSDGGKGPLVNINTATLEELQGISGV
GPSKAEAIVAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=963839 NSQ83_RS12965 WP_198878617.1 2519257..2519874(-) (comEA) [Bacillus sp. PS217]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTAAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCCGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTACCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGACCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCGGGGGTG
GGGCCATCCAAAGCTGAAGCTATTGTTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.073

100

0.971
  comEA Staphylococcus aureus MW2

37.727

100

0.405
  comEA Staphylococcus aureus N315

37.273

100

0.4