Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACJEH4_RS06395 Genome accession   NZ_CP174212
Coordinates   1418826..1420310 (-) Length   494 a.a.
NCBI ID   WP_010947050.1    Uniprot ID   Q5ZVW9
Organism   Legionella pneumophila strain SPF658     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1413826..1425310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEH4_RS06380 (ACJEH4_06380) ravT 1414160..1415182 (-) 1023 WP_010947047.1 Dot/Icm T4SS effector RavT -
  ACJEH4_RS06385 (ACJEH4_06385) ravW 1415274..1416161 (-) 888 WP_010947048.1 Dot/Icm T4SS effector RavW -
  ACJEH4_RS06390 (ACJEH4_06390) - 1416711..1418693 (+) 1983 WP_010947049.1 hypothetical protein -
  ACJEH4_RS06395 (ACJEH4_06395) pilB 1418826..1420310 (-) 1485 WP_010947050.1 GspE family T2SS ATPase variant LspE Machinery gene
  ACJEH4_RS06400 (ACJEH4_06400) lspD 1420314..1422689 (-) 2376 WP_010947051.1 GspD family T2SS secretin variant LspD -
  ACJEH4_RS06405 (ACJEH4_06405) - 1422862..1423890 (+) 1029 WP_015444554.1 Gfo/Idh/MocA family protein -

Sequence

Protein


Download         Length: 494 a.a.        Molecular weight: 55151.48 Da        Isoelectric Point: 5.4872

>NTDB_id=962280 ACJEH4_RS06395 WP_010947050.1 1418826..1420310(-) (pilB) [Legionella pneumophila strain SPF658]
MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPNTPLQAFAEIKRLLQCELDLKQVDESTFQQHLANIYQSKSSILD
AAEGMEEDMDLSMLASQLPVSEDLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPEHTLKTVR
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVV
MIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHELRQEE
KELMGLKPDADVSQVFEPKGCDYCNHLGYKGRTGIYELIIVDETLRGMIHRNENIQTMENYLRPTTPSIREDGFKRVLCG
DTSLAEILRVTSQN

Nucleotide


Download         Length: 1485 bp        

>NTDB_id=962280 ACJEH4_RS06395 WP_010947050.1 1418826..1420310(-) (pilB) [Legionella pneumophila strain SPF658]
ATGGAAAATGAAGAAAAATCACTTAAAATTCCTTACAGTTTTGCCAAAAGCAATGGAATTGTTGTGGGAGAAATACAAGA
AAATCTGGCTATTGTCTATCATTTACCTAACACACCACTGCAGGCTTTCGCTGAGATAAAAAGATTATTGCAATGCGAAC
TTGATCTAAAACAAGTCGATGAATCCACTTTTCAGCAACATTTAGCCAATATTTACCAATCTAAATCTTCAATACTGGAT
GCCGCAGAAGGCATGGAAGAAGATATGGATTTATCCATGTTGGCCAGTCAACTTCCAGTCAGTGAAGACTTATTGGAAAA
CCAAGACGATGCCCCAATTATCCGCTTACTAAACGCTCTGTTTACCCAAGCGATTAAACAAAAAGCATCTGATATACACA
TTGAAACTTATGAAGACAGAGTATTGGTGCGCAATCGAATTGATGGTGTTCTGCAAGAGGTCCTTGAAATTCAACGAGCT
ATCGCTCCTTTGGTCATTTCCAGAGTTAAGGTGATGGCCAAATTGGATATCGCCGAAAAGAGAATTCCTCAAGATGGTCG
AATTTCATTGCGTATAGGAGGACACAATATTGATGTCAGGGTCTCAACTTTACCCTCAAACCATGGAGAGCGAGTGGTTT
TAAGGATATTGGATAAACAAGCTGCTCAACTGGATTTAAATCTTTTGGGTATGCCTGAACATACATTAAAAACAGTACGA
CAAATGATTGCTGAACCTCATGGCATTATTTTAGTCACTGGCCCAACAGGTTCAGGCAAGACAACATCTCTCTATGCTAT
GCTCACCGAACTAAATCAGGTGACTCGTAATATTTTAACCATTGAAGATCCCATTGAATATGACCTGCCTGGTGTAGGAC
AAACGCAGGTTAATACTAAAGTTCAAATGACTTTTGCCAAAGGATTAAGGGCTATATTACGTCAAGATCCGGATGTTGTC
ATGATAGGAGAAATCCGTGATTTGGAAACGGCTGAAATTGCTGTTCAAGCCAGCTTAACAGGGCATTTGGTACTATCAAC
CCTTCATACCAATACCGCATTGGGAGCATTAACTCGCCTGCATGATATGGGAGTTGAGTCTTTTTTATTGTCATCCAGTA
TAGTCGGCCTCATTGCACAACGTTTGGTAAGGAAATTATGCTCTCATTGCAAAACACCCCATGAATTAAGACAGGAAGAA
AAGGAGTTAATGGGCCTTAAACCTGATGCTGATGTATCTCAGGTCTTTGAACCCAAGGGATGCGACTATTGCAACCACTT
GGGTTATAAGGGAAGAACAGGAATTTATGAATTAATTATCGTAGATGAAACCCTGAGAGGAATGATTCATCGTAATGAAA
ATATTCAAACGATGGAAAATTATTTACGACCCACCACTCCAAGCATCCGTGAAGATGGTTTTAAAAGAGTATTATGTGGC
GATACGTCACTTGCAGAAATTTTAAGAGTAACCTCTCAAAACTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(119-385)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5ZVW9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.067

96.356

0.415
  pilB Vibrio campbellii strain DS40M4

46.305

82.186

0.381
  pilB Acinetobacter baumannii D1279779

44.952

84.211

0.379
  pilB Vibrio parahaemolyticus RIMD 2210633

46.04

81.781

0.377
  pilB Acinetobacter baylyi ADP1

47.558

78.745

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

41.475

87.854

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.524

79.15

0.36