Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACJED3_RS20615 Genome accession   NZ_CP174155
Coordinates   3878884..3879501 (+) Length   205 a.a.
NCBI ID   WP_014480312.1    Uniprot ID   -
Organism   Bacillus subtilis strain G01     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3873884..3884501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJED3_RS20580 aroE 3874542..3875384 (+) 843 WP_014480317.1 shikimate dehydrogenase -
  ACJED3_RS20585 yhbY 3875378..3875668 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  ACJED3_RS20590 nadD 3875680..3876249 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  ACJED3_RS20595 yqeK 3876239..3876799 (+) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACJED3_RS20600 rsfS 3876817..3877173 (+) 357 WP_014480315.1 ribosome silencing factor -
  ACJED3_RS20605 yqeM 3877170..3877913 (+) 744 WP_014480314.1 class I SAM-dependent methyltransferase -
  ACJED3_RS20610 comER 3877979..3878800 (-) 822 WP_014480313.1 late competence protein ComER -
  ACJED3_RS20615 comEA 3878884..3879501 (+) 618 WP_014480312.1 competence protein ComEA Machinery gene
  ACJED3_RS20620 comEB 3879568..3880137 (+) 570 WP_003229978.1 ComE operon protein 2 -
  ACJED3_RS20625 comEC 3880141..3882471 (+) 2331 WP_033881047.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACJED3_RS20630 yqzM 3882511..3882645 (-) 135 WP_003229983.1 YqzM family protein -
  ACJED3_RS20635 - 3882686..3882835 (+) 150 WP_003229985.1 hypothetical protein -
  ACJED3_RS20640 holA 3882875..3883918 (+) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  ACJED3_RS20645 rpsT 3883933..3884199 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21781.51 Da        Isoelectric Point: 4.7220

>NTDB_id=962033 ACJED3_RS20615 WP_014480312.1 3878884..3879501(+) (comEA) [Bacillus subtilis strain G01]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=962033 ACJED3_RS20615 WP_014480312.1 3878884..3879501(+) (comEA) [Bacillus subtilis strain G01]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCAG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.512

100

0.995
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39