Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   ACJED3_RS03085 Genome accession   NZ_CP174155
Coordinates   549058..551004 (-) Length   648 a.a.
NCBI ID   WP_014479763.1    Uniprot ID   -
Organism   Bacillus subtilis strain G01     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 544058..556004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJED3_RS03055 yloU 545720..546082 (-) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -
  ACJED3_RS03060 rpmB 546359..546547 (+) 189 WP_003221548.1 50S ribosomal protein L28 -
  ACJED3_RS03065 spoVM 546620..546700 (-) 81 WP_003221545.1 stage V sporulation protein SpoVM -
  ACJED3_RS03070 thiN 546772..547416 (-) 645 WP_014479765.1 thiamine diphosphokinase -
  ACJED3_RS03075 rpe 547489..548142 (-) 654 WP_003232058.1 ribulose-phosphate 3-epimerase -
  ACJED3_RS03080 rsgA 548147..549043 (-) 897 WP_014479764.1 ribosome small subunit-dependent GTPase A -
  ACJED3_RS03085 stkP 549058..551004 (-) 1947 WP_014479763.1 serine/threonine protein kinase PrkC Regulator
  ACJED3_RS03090 prpC 550998..551762 (-) 765 WP_014479762.1 protein-serine/threonine phosphatase PrpC -
  ACJED3_RS03095 rlmN 551769..552860 (-) 1092 WP_014476780.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACJED3_RS03100 rsmB 552864..554207 (-) 1344 WP_014479761.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  ACJED3_RS03105 fmt 554194..555147 (-) 954 WP_014479760.1 methionyl-tRNA formyltransferase -
  ACJED3_RS03110 def 555152..555634 (-) 483 WP_014479759.1 peptide deformylase -

Sequence

Protein


Download         Length: 648 a.a.        Molecular weight: 71894.59 Da        Isoelectric Point: 4.7871

>NTDB_id=961918 ACJED3_RS03085 WP_014479763.1 549058..551004(-) (stkP) [Bacillus subtilis strain G01]
MLIGKRISGRYQILRVIGGGGMANVYLAEDIILDREVAIKILRFDYANDNEFIRRFRREAQSASSLDHPNIVSIYDLGEE
DDIYYIVMEYVEGMTLKEYITANGPLHPKEALNIMEQIVSAIAHAHQNQIVHRDIKPHNILIDHMGNIKVTDFGIATALS
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGRIPFDGESAVSIALKHLQAETPSAKRWNPSVPQS
VENIILKATAKDPFHRYETAEDMEADIKTAFDADRLNEKRFTIQEDEEMTKAIPIIKDEELAKAAGEKEAEVTTAQENKT
KKNGKRKKWPWVLLTICLVFITAGILAVTVFPSLFMPKDVKIPDVSGMEYEKAAGLLEKEGLQVDSELLEISDEKIEEGL
MVKTDPKADTTVKEGATVTLYKSTGKAKTEIGDVTGQTVDQAKKALKDQGFKHVTVNEVNDEKNAGTVIDQNPSAGTELV
PSEDQVKLTVSIGPEDITLRDLKTYSKEAASGYLEDNGLKLVEKEAYSDDVPEGQVVKQKPAAGTAVKPGNEVEVTFSLG
PEKKPAKTVKEKVKIPYEPENEGDELQVQIAVDDADHSISDTYEEFKIKEPTERTIELKIEPGQKGYYQVMVNNKVVSYK
TIEYPKDE

Nucleotide


Download         Length: 1947 bp        

>NTDB_id=961918 ACJED3_RS03085 WP_014479763.1 549058..551004(-) (stkP) [Bacillus subtilis strain G01]
GTGCTAATCGGCAAGCGGATCAGCGGGCGTTACCAAATTCTCCGCGTTATAGGCGGCGGGGGAATGGCCAACGTGTATTT
AGCTGAGGATATCATTCTAGACCGTGAAGTCGCAATTAAAATCCTGCGGTTTGACTATGCAAATGACAATGAATTTATCA
GACGTTTCCGCAGAGAAGCCCAATCCGCATCAAGCCTCGATCACCCGAATATTGTCAGCATTTACGATTTGGGCGAGGAA
GATGATATTTATTATATTGTCATGGAATACGTTGAAGGCATGACGCTTAAAGAATACATAACAGCAAATGGGCCACTTCA
CCCTAAAGAAGCGCTGAACATCATGGAGCAAATAGTCTCAGCCATCGCTCATGCCCATCAAAACCAGATTGTTCACAGAG
ACATCAAGCCGCATAACATTTTGATTGATCATATGGGAAATATCAAAGTAACGGATTTTGGAATTGCGACGGCACTAAGT
TCGACCACAATCACCCATACCAATTCAGTTCTGGGCTCGGTCCATTACTTATCACCTGAACAGGCCCGGGGCGGCTTAGC
CACAAAGAAATCGGATATTTATGCGCTTGGAATCGTTCTATTTGAGCTTTTAACCGGCCGTATTCCGTTTGATGGAGAGT
CAGCAGTCAGCATCGCCTTGAAGCATCTTCAAGCGGAAACTCCTTCGGCAAAAAGGTGGAATCCATCGGTCCCCCAAAGC
GTTGAAAACATCATACTCAAGGCAACTGCCAAAGATCCTTTTCATCGCTACGAAACGGCTGAAGACATGGAAGCAGACAT
AAAAACAGCTTTTGATGCCGACAGACTCAATGAAAAGAGATTTACGATTCAAGAAGATGAAGAAATGACAAAAGCGATAC
CTATCATTAAAGATGAAGAACTCGCTAAAGCTGCTGGCGAAAAAGAAGCTGAAGTGACAACCGCACAAGAAAACAAAACA
AAGAAGAACGGTAAAAGAAAAAAGTGGCCGTGGGTTTTGCTCACGATATGCCTCGTTTTTATCACAGCTGGAATTCTTGC
TGTCACTGTTTTTCCGTCGCTTTTCATGCCTAAGGATGTCAAAATACCTGATGTCTCCGGAATGGAATACGAAAAAGCCG
CAGGGCTCTTGGAAAAAGAAGGTTTACAGGTTGATTCCGAGTTGTTGGAAATCTCAGATGAAAAAATTGAAGAGGGCCTG
ATGGTAAAAACGGATCCTAAAGCGGATACCACAGTCAAAGAAGGCGCCACGGTCACCCTTTATAAGAGCACCGGAAAAGC
AAAAACGGAGATCGGTGATGTGACAGGCCAAACGGTCGACCAGGCAAAAAAAGCGTTGAAGGACCAAGGGTTTAAACATG
TAACAGTAAATGAAGTGAATGACGAGAAAAATGCGGGCACTGTCATTGACCAAAATCCTTCAGCAGGGACTGAGCTTGTC
CCGAGTGAAGATCAAGTCAAACTTACAGTCAGTATCGGACCCGAAGACATTACGCTTAGAGACTTGAAAACCTATAGTAA
AGAAGCAGCGTCTGGATATCTGGAAGACAACGGATTGAAGCTTGTAGAAAAAGAAGCATACTCAGATGATGTTCCAGAAG
GACAGGTTGTCAAACAAAAACCAGCAGCAGGTACGGCAGTAAAGCCGGGAAACGAAGTTGAAGTGACATTCTCTCTCGGA
CCAGAGAAAAAACCTGCGAAAACAGTGAAAGAAAAGGTCAAGATCCCCTACGAACCAGAAAATGAAGGGGACGAGCTTCA
AGTGCAAATCGCGGTTGACGATGCGGATCACAGCATCTCTGACACTTACGAAGAATTTAAGATAAAAGAGCCGACTGAAC
GAACGATCGAACTAAAGATTGAACCCGGCCAAAAAGGGTACTATCAAGTAATGGTAAACAATAAAGTTGTCAGCTACAAA
ACCATTGAGTATCCGAAAGATGAATAA

Domains

Predicted by InterProScan.

Protein kinase domain (Pkinase)

(11-261)

PASTA domain (PASTA)

(430-491)

PASTA domain (PASTA)

(498-558)

PASTA domain (PASTA)

(360-423)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

40.517

89.506

0.363
  stkP Streptococcus pneumoniae D39

40.345

89.506

0.361
  stkP Streptococcus pneumoniae R6

40.345

89.506

0.361