Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   WKI56_RS12365 Genome accession   NZ_CP149650
Coordinates   2484496..2485110 (-) Length   204 a.a.
NCBI ID   WP_017418180.1    Uniprot ID   -
Organism   Bacillus velezensis strain MJ02     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2479496..2490110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKI56_RS12335 (WKI56_12335) rpsT 2479799..2480065 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  WKI56_RS12340 (WKI56_12340) holA 2480028..2481123 (-) 1096 Protein_2390 DNA polymerase III subunit delta -
  WKI56_RS12345 (WKI56_12345) - 2481163..2481314 (-) 152 Protein_2391 hypothetical protein -
  WKI56_RS12350 (WKI56_12350) - 2481355..2481489 (+) 135 WP_003152870.1 YqzM family protein -
  WKI56_RS12355 (WKI56_12355) comEC 2481508..2483859 (-) 2352 WP_017418179.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  WKI56_RS12360 (WKI56_12360) - 2483860..2484429 (-) 570 WP_003152868.1 ComE operon protein 2 -
  WKI56_RS12365 (WKI56_12365) comEA 2484496..2485110 (-) 615 WP_017418180.1 helix-hairpin-helix domain-containing protein Machinery gene
  WKI56_RS12370 (WKI56_12370) comER 2485169..2485990 (+) 822 WP_014305449.1 late competence protein ComER -
  WKI56_RS12375 (WKI56_12375) - 2486059..2486796 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  WKI56_RS12380 (WKI56_12380) rsfS 2486793..2487149 (-) 357 WP_003152864.1 ribosome silencing factor -
  WKI56_RS12385 (WKI56_12385) yqeK 2487146..2487727 (-) 582 WP_017418181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  WKI56_RS12390 (WKI56_12390) - 2487717..2488286 (-) 570 WP_017418182.1 nicotinate-nucleotide adenylyltransferase -
  WKI56_RS12395 (WKI56_12395) yhbY 2488297..2488587 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  WKI56_RS12400 (WKI56_12400) aroE 2488581..2489423 (-) 843 WP_017418183.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22218.33 Da        Isoelectric Point: 5.4841

>NTDB_id=960365 WKI56_RS12365 WP_017418180.1 2484496..2485110(-) (comEA) [Bacillus velezensis strain MJ02]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=960365 WKI56_RS12365 WP_017418180.1 2484496..2485110(-) (comEA) [Bacillus velezensis strain MJ02]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGTGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363