NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	WKI54_RS20890	Genome accession	NZ_CP149648
Coordinates	4048112..4048726 (-)	Length	204 a.a.
NCBI ID	WP_013352942.1	Uniprot ID	A0A411A874
Organism	Bacillus velezensis strain MJ04
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4043112..4053726

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WKI54_RS20860 (WKI54_20860)	rpsT	4043412..4043678 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
WKI54_RS20865 (WKI54_20865)	holA	4043695..4044736 (-)	1042	Protein_4055	DNA polymerase III subunit delta	-
WKI54_RS20870 (WKI54_20870)	-	4044776..4044930 (-)	155	Protein_4056	hypothetical protein	-
WKI54_RS20875 (WKI54_20875)	-	4044971..4045105 (+)	135	WP_003152870.1	YqzM family protein	-
WKI54_RS20880 (WKI54_20880)	comEC	4045124..4047475 (-)	2352	WP_326124076.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
WKI54_RS20885 (WKI54_20885)	-	4047476..4048045 (-)	570	WP_003152868.1	ComE operon protein 2	-
WKI54_RS20890 (WKI54_20890)	comEA	4048112..4048726 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
WKI54_RS20895 (WKI54_20895)	comER	4048785..4049606 (+)	822	WP_013352943.1	late competence protein ComER	-
WKI54_RS20900 (WKI54_20900)	-	4049675..4050412 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
WKI54_RS20905 (WKI54_20905)	rsfS	4050409..4050765 (-)	357	WP_003152864.1	ribosome silencing factor	-
WKI54_RS20910 (WKI54_20910)	yqeK	4050762..4051343 (-)	582	WP_017418181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
WKI54_RS20915 (WKI54_20915)	-	4051333..4051902 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
WKI54_RS20920 (WKI54_20920)	yhbY	4051913..4052203 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
WKI54_RS20925 (WKI54_20925)	aroE	4052197..4053039 (-)	843	WP_017418183.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22190.27 Da Isoelectric Point: 5.4841

                                >NTDB_id=960314 WKI54_RS20890 WP_013352942.1 4048112..4048726(-) (comEA) [Bacillus velezensis strain MJ04]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=960314 WKI54_RS20890 WP_013352942.1 4048112..4048726(-) (comEA) [Bacillus velezensis strain MJ04]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCCT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0960314