Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   WKI54_RS04660 Genome accession   NZ_CP149648
Coordinates   862779..864164 (-) Length   461 a.a.
NCBI ID   WP_339075853.1    Uniprot ID   -
Organism   Bacillus velezensis strain MJ04     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 857779..869164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKI54_RS04625 (WKI54_04625) flgL 857954..858871 (-) 918 WP_270352938.1 flagellar hook-associated protein FlgL -
  WKI54_RS04630 (WKI54_04630) flgK 858883..860400 (-) 1518 WP_014419008.1 flagellar hook-associated protein FlgK -
  WKI54_RS04635 (WKI54_04635) - 860416..860898 (-) 483 WP_003151394.1 flagellar protein FlgN -
  WKI54_RS04640 (WKI54_04640) flgM 860913..861179 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  WKI54_RS04645 (WKI54_04645) - 861249..861668 (-) 420 WP_014419009.1 TIGR03826 family flagellar region protein -
  WKI54_RS04650 (WKI54_04650) comFC 861742..862431 (-) 690 WP_217425079.1 phosphoribosyltransferase family protein Machinery gene
  WKI54_RS04655 (WKI54_04655) - 862437..862721 (-) 285 WP_017418816.1 competence protein ComFB -
  WKI54_RS04660 (WKI54_04660) comFA 862779..864164 (-) 1386 WP_339075853.1 DEAD/DEAH box helicase Machinery gene
  WKI54_RS04665 (WKI54_04665) - 864272..865120 (-) 849 WP_003151387.1 DegV family protein -
  WKI54_RS04670 (WKI54_04670) degU 865218..865907 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  WKI54_RS04675 (WKI54_04675) degS 865984..867147 (-) 1164 WP_003151385.1 two-component sensor histidine kinase DegS Regulator
  WKI54_RS04680 (WKI54_04680) - 867370..868017 (+) 648 WP_339075854.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52534.97 Da        Isoelectric Point: 10.0886

>NTDB_id=960257 WKI54_RS04660 WP_339075853.1 862779..864164(-) (comFA) [Bacillus velezensis strain MJ04]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRIKRFTEKEYITAEPSIIRRKNRYVCQRCGQSDQASFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYSWNQIPENSWEPVKLSWEGTLTDGQKRAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
CQKLQSQLPVFLFVPSVDVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRDGLLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGMTNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=960257 WKI54_RS04660 WP_339075853.1 862779..864164(-) (comFA) [Bacillus velezensis strain MJ04]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
CTCAGACCAGCGTATCAAGCGTTTTACAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCAGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAATCCCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTCTCTGCTTTGTACGGCGGCAGCGAAGACAAAGGCCGCCTGACTCCGCTGATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTT
TGCCAAAAACTTCAATCACAGCTGCCTGTTTTCTTATTCGTTCCGTCTGTAGACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCTGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGAGTTACCGTACCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATGACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(325-421)

Type III restriction enzyme, res subunit (ResIII)

(118-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.609

99.783

0.625