Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   WDR05_RS13235 Genome accession   NZ_CP149444
Coordinates   2543428..2544045 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain HC-9     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2538428..2549045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDR05_RS13205 (WDR05_13195) rpsT 2538737..2539003 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  WDR05_RS13210 (WDR05_13200) holA 2539018..2540061 (-) 1044 WP_015714286.1 DNA polymerase III subunit delta -
  WDR05_RS13215 (WDR05_13205) - 2540101..2540250 (-) 150 WP_003229985.1 hypothetical protein -
  WDR05_RS13220 (WDR05_13210) yqzM 2540291..2540425 (+) 135 WP_003229983.1 YqzM family protein -
  WDR05_RS13225 (WDR05_13215) comEC 2540465..2542795 (-) 2331 WP_074794436.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  WDR05_RS13230 (WDR05_13220) comEB 2542792..2543361 (-) 570 WP_015714288.1 ComE operon protein 2 -
  WDR05_RS13235 (WDR05_13225) comEA 2543428..2544045 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  WDR05_RS13240 (WDR05_13230) comER 2544129..2544950 (+) 822 WP_004398597.1 late competence protein ComER -
  WDR05_RS13245 (WDR05_13235) yqeM 2545016..2545759 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  WDR05_RS13250 (WDR05_13240) rsfS 2545756..2546112 (-) 357 WP_003229971.1 ribosome silencing factor -
  WDR05_RS13255 (WDR05_13245) yqeK 2546130..2546690 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  WDR05_RS13260 (WDR05_13250) nadD 2546680..2547249 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  WDR05_RS13265 (WDR05_13255) yhbY 2547261..2547551 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  WDR05_RS13270 (WDR05_13260) aroE 2547545..2548387 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=959135 WDR05_RS13235 WP_004398514.1 2543428..2544045(-) (comEA) [Bacillus subtilis strain HC-9]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=959135 WDR05_RS13235 WP_004398514.1 2543428..2544045(-) (comEA) [Bacillus subtilis strain HC-9]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39