Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   Q6225_RS14730 Genome accession   NZ_CP148900
Coordinates   3150762..3151355 (-) Length   197 a.a.
NCBI ID   WP_033907305.1    Uniprot ID   A0AAW9B9Y8
Organism   Vibrio alginolyticus strain I1B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3145762..3156355
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q6225_RS14710 (Q6225_14705) aroB 3146647..3147747 (-) 1101 WP_104975646.1 3-dehydroquinate synthase -
  Q6225_RS14715 (Q6225_14710) aroK 3147780..3148298 (-) 519 WP_005381319.1 shikimate kinase AroK -
  Q6225_RS14720 (Q6225_14715) pilQ 3148486..3150231 (-) 1746 WP_165439071.1 type IV pilus secretin PilQ family protein Machinery gene
  Q6225_RS14725 (Q6225_14720) pilP 3150257..3150772 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  Q6225_RS14730 (Q6225_14725) pilO 3150762..3151355 (-) 594 WP_033907305.1 type 4a pilus biogenesis protein PilO Machinery gene
  Q6225_RS14735 (Q6225_14730) pilN 3151348..3151923 (-) 576 WP_104975648.1 PilN domain-containing protein Machinery gene
  Q6225_RS14740 (Q6225_14735) pilM 3151907..3152923 (-) 1017 WP_005381324.1 type IV pilus assembly protein PilM Machinery gene
  Q6225_RS14745 (Q6225_14740) - 3153068..3155629 (+) 2562 WP_025768081.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23001.51 Da        Isoelectric Point: 5.0948

>NTDB_id=956140 Q6225_RS14730 WP_033907305.1 3150762..3151355(-) (pilO) [Vibrio alginolyticus strain I1B]
MVDWQDLEIDEMAEWPLLPQIVVIALLIMAIQGLGYWFYLKPKEEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=956140 Q6225_RS14730 WP_033907305.1 3150762..3151355(-) (pilO) [Vibrio alginolyticus strain I1B]
GTGGTTGATTGGCAGGATCTCGAAATAGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTGTAGTAATCGCGCTTCT
GATCATGGCGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGAAGAACAGTTGAATCACTTGATCCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACTCGTTACGAGTTATTACTGCAACAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
GCTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTTC
CTTTAAACATAGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGTGAAACCCTGCACTTTCGGGTGCGCGCTAACACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

87.895

96.447

0.848
  pilO Vibrio cholerae strain A1552

70.681

96.954

0.685