Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   Q6225_RS14740 Genome accession   NZ_CP148900
Coordinates   3151907..3152923 (-) Length   338 a.a.
NCBI ID   WP_005381324.1    Uniprot ID   A0AAW9BNQ7
Organism   Vibrio alginolyticus strain I1B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3146907..3157923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q6225_RS14715 (Q6225_14710) aroK 3147780..3148298 (-) 519 WP_005381319.1 shikimate kinase AroK -
  Q6225_RS14720 (Q6225_14715) pilQ 3148486..3150231 (-) 1746 WP_165439071.1 type IV pilus secretin PilQ family protein Machinery gene
  Q6225_RS14725 (Q6225_14720) pilP 3150257..3150772 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  Q6225_RS14730 (Q6225_14725) pilO 3150762..3151355 (-) 594 WP_033907305.1 type 4a pilus biogenesis protein PilO Machinery gene
  Q6225_RS14735 (Q6225_14730) pilN 3151348..3151923 (-) 576 WP_104975648.1 PilN domain-containing protein Machinery gene
  Q6225_RS14740 (Q6225_14735) pilM 3151907..3152923 (-) 1017 WP_005381324.1 type IV pilus assembly protein PilM Machinery gene
  Q6225_RS14745 (Q6225_14740) - 3153068..3155629 (+) 2562 WP_025768081.1 PBP1A family penicillin-binding protein -
  Q6225_RS14750 (Q6225_14745) oxyR 3155682..3156593 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  Q6225_RS14755 (Q6225_14750) - 3156763..3157491 (+) 729 WP_005382781.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37581.92 Da        Isoelectric Point: 7.2224

>NTDB_id=956142 Q6225_RS14740 WP_005381324.1 3151907..3152923(-) (pilM) [Vibrio alginolyticus strain I1B]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGANSASPVDFLSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=956142 Q6225_RS14740 WP_005381324.1 3151907..3152923(-) (pilM) [Vibrio alginolyticus strain I1B]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAAAAAAGTTGCGATTTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGCAAACAGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCGTAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAAGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTAGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

83.136

100

0.831
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503