Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACF2JY_RS07735 Genome accession   NZ_CP172966
Coordinates   1468509..1469126 (+) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   P39694
Organism   Bacillus subtilis strain cel     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1463509..1474126
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF2JY_RS07700 (ACF2JY_07700) aroE 1464167..1465009 (+) 843 WP_041351695.1 shikimate dehydrogenase -
  ACF2JY_RS07705 (ACF2JY_07705) yhbY 1465003..1465293 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  ACF2JY_RS07710 (ACF2JY_07710) nadD 1465305..1465874 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  ACF2JY_RS07715 (ACF2JY_07715) yqeK 1465864..1466424 (+) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACF2JY_RS07720 (ACF2JY_07720) rsfS 1466442..1466798 (+) 357 WP_014480315.1 ribosome silencing factor -
  ACF2JY_RS07725 (ACF2JY_07725) yqeM 1466795..1467538 (+) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  ACF2JY_RS07730 (ACF2JY_07730) comER 1467604..1468425 (-) 822 WP_014480313.1 late competence protein ComER -
  ACF2JY_RS07735 (ACF2JY_07735) comEA 1468509..1469126 (+) 618 WP_004398514.1 competence protein ComEA Machinery gene
  ACF2JY_RS07740 (ACF2JY_07740) comEB 1469193..1469762 (+) 570 WP_046381196.1 ComE operon protein 2 -
  ACF2JY_RS07745 (ACF2JY_07745) comEC 1469766..1472096 (+) 2331 WP_046381195.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACF2JY_RS07750 (ACF2JY_07750) yqzM 1472136..1472270 (-) 135 WP_003229983.1 YqzM family protein -
  ACF2JY_RS07755 (ACF2JY_07755) - 1472311..1472460 (+) 150 WP_003229985.1 hypothetical protein -
  ACF2JY_RS07760 (ACF2JY_07760) holA 1472500..1473543 (+) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  ACF2JY_RS07765 (ACF2JY_07765) rpsT 1473558..1473824 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=956103 ACF2JY_RS07735 WP_004398514.1 1468509..1469126(+) (comEA) [Bacillus subtilis strain cel]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=956103 ACF2JY_RS07735 WP_004398514.1 1468509..1469126(+) (comEA) [Bacillus subtilis strain cel]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8DFK

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39