NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	WG927_RS12420	Genome accession	NZ_CP148896
Coordinates	2547778..2548848 (-)	Length	356 a.a.
NCBI ID	WP_014418378.1	Uniprot ID	A0AAP4DGV9
Organism	Bacillus velezensis strain XS142
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2542778..2553848

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WG927_RS12370 (WG927_12355)	sipW	2542837..2543421 (-)	585	WP_012117977.1	signal peptidase I SipW	-
WG927_RS12375 (WG927_12360)	tapA	2543393..2544064 (-)	672	WP_014418371.1	amyloid fiber anchoring/assembly protein TapA	-
WG927_RS12380 (WG927_12365)	-	2544323..2544652 (+)	330	WP_012117979.1	DUF3889 domain-containing protein	-
WG927_RS12385 (WG927_12370)	-	2544693..2544872 (-)	180	WP_003153093.1	YqzE family protein	-
WG927_RS12390 (WG927_12375)	comGG	2544929..2545306 (-)	378	WP_017418138.1	competence type IV pilus minor pilin ComGG	Machinery gene
WG927_RS12395 (WG927_12380)	comGF	2545307..2545702 (-)	396	WP_050569584.1	competence type IV pilus minor pilin ComGF	-
WG927_RS12400 (WG927_12385)	comGE	2545716..2546030 (-)	315	WP_029973875.1	competence type IV pilus minor pilin ComGE	Machinery gene
WG927_RS12405 (WG927_12390)	comGD	2546014..2546451 (-)	438	WP_007612572.1	competence type IV pilus minor pilin ComGD	Machinery gene
WG927_RS12410 (WG927_12395)	comGC	2546441..2546749 (-)	309	WP_014418376.1	competence type IV pilus major pilin ComGC	Machinery gene
WG927_RS12415 (WG927_12400)	comGB	2546754..2547791 (-)	1038	WP_014418377.1	competence type IV pilus assembly protein ComGB	Machinery gene
WG927_RS12420 (WG927_12405)	comGA	2547778..2548848 (-)	1071	WP_014418378.1	competence type IV pilus ATPase ComGA	Machinery gene
WG927_RS12425 (WG927_12410)	-	2549041..2549991 (-)	951	WP_014418379.1	magnesium transporter CorA family protein	-
WG927_RS12430 (WG927_12415)	-	2550137..2551438 (+)	1302	WP_029973873.1	hemolysin family protein	-
WG927_RS12435 (WG927_12420)	-	2551476..2552306 (-)	831	WP_025649847.1	STAS domain-containing protein	-
WG927_RS12445 (WG927_12430)	-	2552513..2552893 (-)	381	WP_003153079.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
WG927_RS12450 (WG927_12435)	-	2553130..2553375 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40529.81 Da Isoelectric Point: 9.3485

                                >NTDB_id=956035 WG927_RS12420 WP_014418378.1 2547778..2548848(-) (comGA) [Bacillus velezensis strain XS142]

MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=956035 WG927_RS12420 WP_014418378.1 2547778..2548848(-) (comGA) [Bacillus velezensis strain XS142]

GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC

GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG

TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT

CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA

AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT

GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG

GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGTGCCGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT

ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC

TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA

GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG

CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCTCTCGGCTATGTCACGACCGACAATTA

CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.303	100	0.823
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0956035