NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	WHO18_RS13880	Genome accession	NZ_CP148128
Coordinates	2640100..2640717 (-)	Length	205 a.a.
NCBI ID	WP_004398514.1	Uniprot ID	A0AAE2SLR2
Organism	Bacillus subtilis isolate FELIX_MS620
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2635100..2645717

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WHO18_RS13850	rpsT	2635402..2635668 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
WHO18_RS13855	holA	2635683..2636726 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
WHO18_RS13860	-	2636766..2636915 (-)	150	WP_003229985.1	hypothetical protein	-
WHO18_RS13865	yqzM	2636956..2637090 (+)	135	WP_003229983.1	YqzM family protein	-
WHO18_RS13870	comEC	2637130..2639460 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
WHO18_RS13875	comEB	2639464..2640033 (-)	570	WP_003229978.1	ComE operon protein 2	-
WHO18_RS13880	comEA	2640100..2640717 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
WHO18_RS13885	comER	2640801..2641622 (+)	822	WP_004398597.1	late competence protein ComER	-
WHO18_RS13890	yqeM	2641688..2642431 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
WHO18_RS13895	rsfS	2642428..2642784 (-)	357	WP_003229971.1	ribosome silencing factor	-
WHO18_RS13900	yqeK	2642802..2643362 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
WHO18_RS13905	nadD	2643352..2643921 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
WHO18_RS13910	yhbY	2643933..2644223 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
WHO18_RS13915	aroE	2644217..2645059 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 205 a.a. Molecular weight: 21769.45 Da Isoelectric Point: 4.7220

                                >NTDB_id=951458 WHO18_RS13880 WP_004398514.1 2640100..2640717(-) (comEA) [Bacillus subtilis isolate FELIX_MS620]

MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT

GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV

GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

Download Length: 618 bp

                                >NTDB_id=951458 WHO18_RS13880 WP_004398514.1 2640100..2640717(-) (comEA) [Bacillus subtilis isolate FELIX_MS620]

ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT

GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA

ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA

GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA

GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG

TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG

GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT

TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39

Detailed information NTG0951458