NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	WHO22_RS13875	Genome accession	NZ_CP148122
Coordinates	2640025..2640642 (-)	Length	205 a.a.
NCBI ID	WP_004398514.1	Uniprot ID	A0AAE2SLR2
Organism	Bacillus subtilis isolate FELIX_MS513
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2635025..2645642

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WHO22_RS13845	rpsT	2635327..2635593 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
WHO22_RS13850	holA	2635608..2636651 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
WHO22_RS13855	-	2636691..2636840 (-)	150	WP_003229985.1	hypothetical protein	-
WHO22_RS13860	yqzM	2636881..2637015 (+)	135	WP_003229983.1	YqzM family protein	-
WHO22_RS13865	comEC	2637055..2639385 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
WHO22_RS13870	comEB	2639389..2639958 (-)	570	WP_003229978.1	ComE operon protein 2	-
WHO22_RS13875	comEA	2640025..2640642 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
WHO22_RS13880	comER	2640726..2641547 (+)	822	WP_004398597.1	late competence protein ComER	-
WHO22_RS13885	yqeM	2641613..2642356 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
WHO22_RS13890	rsfS	2642353..2642709 (-)	357	WP_003229971.1	ribosome silencing factor	-
WHO22_RS13895	yqeK	2642727..2643287 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
WHO22_RS13900	nadD	2643277..2643846 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
WHO22_RS13905	yhbY	2643858..2644148 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
WHO22_RS13910	aroE	2644142..2644984 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 205 a.a. Molecular weight: 21769.45 Da Isoelectric Point: 4.7220

                                >NTDB_id=951240 WHO22_RS13875 WP_004398514.1 2640025..2640642(-) (comEA) [Bacillus subtilis isolate FELIX_MS513]

MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT

GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV

GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

Download Length: 618 bp

                                >NTDB_id=951240 WHO22_RS13875 WP_004398514.1 2640025..2640642(-) (comEA) [Bacillus subtilis isolate FELIX_MS513]

ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT

GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA

ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA

GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA

GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG

TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG

GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT

TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39

Detailed information NTG0951240