Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   ACHP5H_RS03535 Genome accession   NZ_CP172194
Coordinates   734315..734974 (-) Length   219 a.a.
NCBI ID   WP_001221486.1    Uniprot ID   -
Organism   Escherichia coli strain GF66.1     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 729315..739974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHP5H_RS03510 (ACHP5H_03510) parE 729367..731259 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  ACHP5H_RS03515 (ACHP5H_03515) ygiN 731307..731621 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  ACHP5H_RS03520 (ACHP5H_03520) mdaB 731652..732233 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  ACHP5H_RS03525 (ACHP5H_03525) ygiZ 732591..732923 (+) 333 WP_000912091.1 DUF2645 family protein -
  ACHP5H_RS03530 (ACHP5H_03530) qseC 732969..734318 (-) 1350 WP_000673348.1 quorum sensing histidine kinase QseC -
  ACHP5H_RS03535 (ACHP5H_03535) ciaR 734315..734974 (-) 660 WP_001221486.1 quorum sensing response regulator transcription factor QseB Regulator
  ACHP5H_RS03540 (ACHP5H_03540) ygiW 735126..735518 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  ACHP5H_RS03545 (ACHP5H_03545) ygiV 735571..736053 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  ACHP5H_RS03550 (ACHP5H_03550) ygiS 736162..737769 (+) 1608 WP_040234936.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24692.61 Da        Isoelectric Point: 6.3527

>NTDB_id=951031 ACHP5H_RS03535 WP_001221486.1 734315..734974(-) (ciaR) [Escherichia coli strain GF66.1]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGCQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=951031 ACHP5H_RS03535 WP_001221486.1 734315..734974(-) (ciaR) [Escherichia coli strain GF66.1]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTTGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365