NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	WHO62_RS13855	Genome accession	NZ_CP148114
Coordinates	2639518..2640135 (-)	Length	205 a.a.
NCBI ID	WP_004398514.1	Uniprot ID	A0AAE2SLR2
Organism	Bacillus subtilis isolate FELIX_MS506
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2634518..2645135

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WHO62_RS13825	rpsT	2634820..2635086 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
WHO62_RS13830	holA	2635101..2636144 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
WHO62_RS13835	-	2636184..2636333 (-)	150	WP_003229985.1	hypothetical protein	-
WHO62_RS13840	yqzM	2636374..2636508 (+)	135	WP_003229983.1	YqzM family protein	-
WHO62_RS13845	comEC	2636548..2638878 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
WHO62_RS13850	comEB	2638882..2639451 (-)	570	WP_003229978.1	ComE operon protein 2	-
WHO62_RS13855	comEA	2639518..2640135 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
WHO62_RS13860	comER	2640219..2641040 (+)	822	WP_004398597.1	late competence protein ComER	-
WHO62_RS13865	yqeM	2641106..2641849 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
WHO62_RS13870	rsfS	2641846..2642202 (-)	357	WP_003229971.1	ribosome silencing factor	-
WHO62_RS13875	yqeK	2642220..2642780 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
WHO62_RS13880	nadD	2642770..2643339 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
WHO62_RS13885	yhbY	2643351..2643641 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
WHO62_RS13890	aroE	2643635..2644477 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 205 a.a. Molecular weight: 21769.45 Da Isoelectric Point: 4.7220

                                >NTDB_id=950938 WHO62_RS13855 WP_004398514.1 2639518..2640135(-) (comEA) [Bacillus subtilis isolate FELIX_MS506]

MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT

GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV

GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

Download Length: 618 bp

                                >NTDB_id=950938 WHO62_RS13855 WP_004398514.1 2639518..2640135(-) (comEA) [Bacillus subtilis isolate FELIX_MS506]

ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT

GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA

ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA

GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA

GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG

TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG

GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT

TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39

Detailed information NTG0950938