NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	WHO49_RS13855	Genome accession	NZ_CP148110
Coordinates	2639743..2640360 (-)	Length	205 a.a.
NCBI ID	WP_004398514.1	Uniprot ID	A0AAE2SLR2
Organism	Bacillus subtilis isolate FELIX_MS499
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2634743..2645360

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WHO49_RS13825	rpsT	2635045..2635311 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
WHO49_RS13830	holA	2635326..2636369 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
WHO49_RS13835	-	2636409..2636558 (-)	150	WP_003229985.1	hypothetical protein	-
WHO49_RS13840	yqzM	2636599..2636733 (+)	135	WP_003229983.1	YqzM family protein	-
WHO49_RS13845	comEC	2636773..2639103 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
WHO49_RS13850	comEB	2639107..2639676 (-)	570	WP_003229978.1	ComE operon protein 2	-
WHO49_RS13855	comEA	2639743..2640360 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
WHO49_RS13860	comER	2640444..2641265 (+)	822	WP_004398597.1	late competence protein ComER	-
WHO49_RS13865	yqeM	2641331..2642074 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
WHO49_RS13870	rsfS	2642071..2642427 (-)	357	WP_003229971.1	ribosome silencing factor	-
WHO49_RS13875	yqeK	2642445..2643005 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
WHO49_RS13880	nadD	2642995..2643564 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
WHO49_RS13885	yhbY	2643576..2643866 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
WHO49_RS13890	aroE	2643860..2644702 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 205 a.a. Molecular weight: 21769.45 Da Isoelectric Point: 4.7220

                                >NTDB_id=950766 WHO49_RS13855 WP_004398514.1 2639743..2640360(-) (comEA) [Bacillus subtilis isolate FELIX_MS499]

MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT

GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV

GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

Download Length: 618 bp

                                >NTDB_id=950766 WHO49_RS13855 WP_004398514.1 2639743..2640360(-) (comEA) [Bacillus subtilis isolate FELIX_MS499]

ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT

GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA

ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA

GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA

GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG

TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG

GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT

TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39

Detailed information NTG0950766