Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   WHL98_RS13870 Genome accession   NZ_CP148104
Coordinates   2641365..2641982 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis subsp. subtilis isolate FELIX_MS438     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2636365..2646982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHL98_RS13840 rpsT 2636667..2636933 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  WHL98_RS13845 holA 2636948..2637991 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  WHL98_RS13850 - 2638031..2638180 (-) 150 WP_003229985.1 hypothetical protein -
  WHL98_RS13855 yqzM 2638221..2638355 (+) 135 WP_003229983.1 YqzM family protein -
  WHL98_RS13860 comEC 2638395..2640725 (-) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  WHL98_RS13865 comEB 2640729..2641298 (-) 570 WP_003229978.1 ComE operon protein 2 -
  WHL98_RS13870 comEA 2641365..2641982 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  WHL98_RS13875 comER 2642066..2642887 (+) 822 WP_004398597.1 late competence protein ComER -
  WHL98_RS13880 yqeM 2642953..2643696 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  WHL98_RS13885 rsfS 2643693..2644049 (-) 357 WP_003229971.1 ribosome silencing factor -
  WHL98_RS13890 yqeK 2644067..2644627 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  WHL98_RS13895 nadD 2644617..2645186 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  WHL98_RS13900 yhbY 2645198..2645488 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  WHL98_RS13905 aroE 2645482..2646324 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=950613 WHL98_RS13870 WP_004398514.1 2641365..2641982(-) (comEA) [Bacillus subtilis subsp. subtilis isolate FELIX_MS438]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=950613 WHL98_RS13870 WP_004398514.1 2641365..2641982(-) (comEA) [Bacillus subtilis subsp. subtilis isolate FELIX_MS438]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39