Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   WHL49_RS12705 Genome accession   NZ_CP148103
Coordinates   2481883..2482500 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis subsp. subtilis isolate FELIX_MS361     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2476883..2487500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHL49_RS12675 rpsT 2477185..2477451 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  WHL49_RS12680 holA 2477466..2478509 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  WHL49_RS12685 - 2478549..2478698 (-) 150 WP_003229985.1 hypothetical protein -
  WHL49_RS12690 yqzM 2478739..2478873 (+) 135 WP_003229983.1 YqzM family protein -
  WHL49_RS12695 comEC 2478913..2481243 (-) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  WHL49_RS12700 comEB 2481247..2481816 (-) 570 WP_003229978.1 ComE operon protein 2 -
  WHL49_RS12705 comEA 2481883..2482500 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  WHL49_RS12710 comER 2482584..2483405 (+) 822 WP_004398597.1 late competence protein ComER -
  WHL49_RS12715 yqeM 2483471..2484214 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  WHL49_RS12720 rsfS 2484211..2484567 (-) 357 WP_003229971.1 ribosome silencing factor -
  WHL49_RS12725 yqeK 2484585..2485145 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  WHL49_RS12730 nadD 2485135..2485704 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  WHL49_RS12735 yhbY 2485716..2486006 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  WHL49_RS12740 aroE 2486000..2486842 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=950527 WHL49_RS12705 WP_004398514.1 2481883..2482500(-) (comEA) [Bacillus subtilis subsp. subtilis isolate FELIX_MS361]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=950527 WHL49_RS12705 WP_004398514.1 2481883..2482500(-) (comEA) [Bacillus subtilis subsp. subtilis isolate FELIX_MS361]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39