NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	WFE12_RS13875	Genome accession	NZ_CP147877
Coordinates	2641924..2642541 (-)	Length	205 a.a.
NCBI ID	WP_004398514.1	Uniprot ID	A0AAE2SLR2
Organism	Bacillus subtilis subsp. subtilis str. 168
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2636924..2647541

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WFE12_RS13845	rpsT	2637226..2637492 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
WFE12_RS13850	holA	2637507..2638550 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
WFE12_RS13855	-	2638590..2638739 (-)	150	WP_003229985.1	hypothetical protein	-
WFE12_RS13860	yqzM	2638780..2638914 (+)	135	WP_003229983.1	YqzM family protein	-
WFE12_RS13865	comEC	2638954..2641284 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
WFE12_RS13870	comEB	2641288..2641857 (-)	570	WP_003229978.1	ComE operon protein 2	-
WFE12_RS13875	comEA	2641924..2642541 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
WFE12_RS13880	comER	2642625..2643446 (+)	822	WP_004398597.1	late competence protein ComER	-
WFE12_RS13885	yqeM	2643512..2644255 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
WFE12_RS13890	rsfS	2644252..2644608 (-)	357	WP_003229971.1	ribosome silencing factor	-
WFE12_RS13895	yqeK	2644626..2645186 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
WFE12_RS13900	nadD	2645176..2645745 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
WFE12_RS13905	yhbY	2645757..2646047 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
WFE12_RS13910	aroE	2646041..2646883 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 205 a.a. Molecular weight: 21769.45 Da Isoelectric Point: 4.7220

                                >NTDB_id=949157 WFE12_RS13875 WP_004398514.1 2641924..2642541(-) (comEA) [Bacillus subtilis subsp. subtilis str. 168]

MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT

GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV

GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

Download Length: 618 bp

                                >NTDB_id=949157 WFE12_RS13875 WP_004398514.1 2641924..2642541(-) (comEA) [Bacillus subtilis subsp. subtilis str. 168]

ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT

GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA

ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA

GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA

GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG

TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG

GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT

TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39

Detailed information NTG0949157