Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   WFE12_RS13430 Genome accession   NZ_CP147877
Coordinates   2560417..2561487 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2555417..2566487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WFE12_RS13385 tapA 2555896..2556657 (-) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -
  WFE12_RS13390 yqzG 2556929..2557255 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  WFE12_RS13395 spoIIT 2557297..2557476 (-) 180 WP_003230176.1 YqzE family protein -
  WFE12_RS13400 comGG 2557547..2557921 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  WFE12_RS13405 comGF 2557922..2558305 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  WFE12_RS13410 comGE 2558331..2558678 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  WFE12_RS13415 comGD 2558662..2559093 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  WFE12_RS13420 comGC 2559083..2559379 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  WFE12_RS13425 comGB 2559393..2560430 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  WFE12_RS13430 comGA 2560417..2561487 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  WFE12_RS13435 corA 2561899..2562852 (-) 954 WP_032723504.1 magnesium transporter CorA -
  WFE12_RS13440 yqhB 2562995..2564323 (+) 1329 WP_009967730.1 hemolysin family protein -
  WFE12_RS13445 rsbRD 2564376..2565212 (-) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -
  WFE12_RS13455 mgsR 2565436..2565816 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  WFE12_RS13460 yqgY 2566048..2566293 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=949153 WFE12_RS13430 WP_004399124.1 2560417..2561487(-) (comGA) [Bacillus subtilis subsp. subtilis str. 168]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=949153 WFE12_RS13430 WP_004399124.1 2560417..2561487(-) (comGA) [Bacillus subtilis subsp. subtilis str. 168]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGTTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACTAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1