Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   WCU05_RS27025 Genome accession   NZ_CP147622
Coordinates   5718903..5720240 (+) Length   445 a.a.
NCBI ID   WP_003094694.1    Uniprot ID   A0A0H2ZG94
Organism   Pseudomonas aeruginosa strain GN06251     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5713903..5725240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU05_RS27000 (WCU05_27000) pgeF 5714046..5714774 (-) 729 WP_003119426.1 peptidoglycan editing factor PgeF -
  WCU05_RS27005 (WCU05_27005) rluD 5714771..5715733 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  WCU05_RS27010 (WCU05_27010) - 5715879..5716904 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  WCU05_RS27015 (WCU05_27015) - 5717061..5717306 (+) 246 WP_003102591.1 PP0621 family protein -
  WCU05_RS27020 (WCU05_27020) pilS 5717296..5718888 (+) 1593 WP_003119425.1 two-component system sensor histidine kinase PilS Regulator
  WCU05_RS27025 (WCU05_27025) pilR 5718903..5720240 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  WCU05_RS27030 (WCU05_27030) thiO 5720286..5721380 (-) 1095 WP_003119424.1 glycine oxidase ThiO -
  WCU05_RS27035 (WCU05_27035) - 5721515..5722024 (+) 510 WP_003102595.1 Tfp pilus assembly protein FimT/FimU -
  WCU05_RS27040 (WCU05_27040) - 5722130..5722636 (+) 507 WP_003102598.1 Tfp pilus assembly protein FimT/FimU -
  WCU05_RS27045 (WCU05_27045) pilV 5722627..5723184 (+) 558 WP_003119423.1 type 4a pilus minor pilin PilV Machinery gene
  WCU05_RS27050 (WCU05_27050) pilW 5723181..5724005 (+) 825 WP_003119422.1 type 4a pilus minor pilin PilW -
  WCU05_RS27055 (WCU05_27055) pilX 5724002..5724589 (+) 588 WP_015648054.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49737.06 Da        Isoelectric Point: 5.8654

>NTDB_id=947685 WCU05_RS27025 WP_003094694.1 5718903..5720240(+) (pilR) [Pseudomonas aeruginosa strain GN06251]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=947685 WCU05_RS27025 WP_003094694.1 5718903..5720240(+) (pilR) [Pseudomonas aeruginosa strain GN06251]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGTAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATAGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-115)

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZG94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

100

100

1
  pilR Acinetobacter baumannii strain A118

51.198

100

0.528
  luxO Vibrio cholerae strain A1552

38.17

100

0.384