Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   WBM82_RS13685 Genome accession   NZ_CP147494
Coordinates   2579222..2579839 (-) Length   205 a.a.
NCBI ID   WP_014480312.1    Uniprot ID   -
Organism   Bacillus subtilis strain YT1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2574222..2584839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WBM82_RS13655 (WBM82_13700) rpsT 2574530..2574796 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  WBM82_RS13660 (WBM82_13705) holA 2574811..2575854 (-) 1044 WP_015714286.1 DNA polymerase III subunit delta -
  WBM82_RS13665 (WBM82_13710) - 2575894..2576037 (-) 144 WP_338816908.1 hypothetical protein -
  WBM82_RS13670 (WBM82_13715) yqzM 2576078..2576212 (+) 135 WP_003229983.1 YqzM family protein -
  WBM82_RS13675 (WBM82_13720) comEC 2576252..2578582 (-) 2331 WP_248719384.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  WBM82_RS13680 (WBM82_13725) comEB 2578586..2579155 (-) 570 WP_003229978.1 ComE operon protein 2 -
  WBM82_RS13685 (WBM82_13730) comEA 2579222..2579839 (-) 618 WP_014480312.1 competence protein ComEA Machinery gene
  WBM82_RS13690 (WBM82_13735) comER 2579923..2580744 (+) 822 WP_014480313.1 late competence protein ComER -
  WBM82_RS13695 (WBM82_13740) yqeM 2580810..2581553 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  WBM82_RS13700 (WBM82_13745) rsfS 2581550..2581906 (-) 357 WP_041333759.1 ribosome silencing factor -
  WBM82_RS13705 (WBM82_13750) yqeK 2581924..2582484 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  WBM82_RS13710 (WBM82_13755) nadD 2582474..2583043 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  WBM82_RS13715 (WBM82_13760) yhbY 2583055..2583345 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  WBM82_RS13720 (WBM82_13765) aroE 2583339..2584181 (-) 843 WP_029317883.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21781.51 Da        Isoelectric Point: 4.7220

>NTDB_id=946435 WBM82_RS13685 WP_014480312.1 2579222..2579839(-) (comEA) [Bacillus subtilis strain YT1]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=946435 WBM82_RS13685 WP_014480312.1 2579222..2579839(-) (comEA) [Bacillus subtilis strain YT1]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.512

100

0.995
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39