Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilH   Type   Machinery gene
Locus tag   ACFW98_RS02050 Genome accession   NZ_CP170719
Coordinates   427554..427916 (+) Length   120 a.a.
NCBI ID   WP_003154238.1    Uniprot ID   A0A150FA43
Organism   Bacillus amyloliquefaciens strain JP5008     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422554..432916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW98_RS02020 (ACFW98_02020) flgD 423458..423898 (+) 441 WP_015239855.1 flagellar hook assembly protein FlgD -
  ACFW98_RS02025 (ACFW98_02025) flgG 423923..424699 (+) 777 WP_007409783.1 flagellar basal body rod protein FlgG -
  ACFW98_RS02030 (ACFW98_02030) - 424739..424954 (+) 216 WP_003154243.1 flagellar FlbD family protein -
  ACFW98_RS02035 (ACFW98_02035) fliL 424951..425370 (+) 420 WP_014417773.1 flagellar basal body-associated protein FliL -
  ACFW98_RS02040 (ACFW98_02040) fliM 425404..426402 (+) 999 WP_003154240.1 flagellar motor switch protein FliM -
  ACFW98_RS02045 (ACFW98_02045) fliY 426392..427527 (+) 1136 Protein_396 flagellar motor switch phosphatase FliY -
  ACFW98_RS02050 (ACFW98_02050) pilH 427554..427916 (+) 363 WP_003154238.1 chemotaxis protein CheY Machinery gene
  ACFW98_RS02055 (ACFW98_02055) fliZ 427931..428584 (+) 654 WP_003154237.1 flagella biosynthesis regulatory protein FliZ -
  ACFW98_RS02060 (ACFW98_02060) fliP 428577..429242 (+) 666 WP_007409786.1 flagellar type III secretion system pore protein FliP -
  ACFW98_RS02065 (ACFW98_02065) fliQ 429257..429526 (+) 270 WP_003154234.1 flagellar biosynthesis protein FliQ -
  ACFW98_RS02070 (ACFW98_02070) fliR 429533..430312 (+) 780 WP_395096749.1 flagellar biosynthetic protein FliR -
  ACFW98_RS02075 (ACFW98_02075) flhB 430312..431391 (+) 1080 WP_007409787.1 flagellar biosynthesis protein FlhB -

Sequence

Protein


Download         Length: 120 a.a.        Molecular weight: 13209.53 Da        Isoelectric Point: 4.6805

>NTDB_id=945137 ACFW98_RS02050 WP_003154238.1 427554..427916(+) (pilH) [Bacillus amyloliquefaciens strain JP5008]
MAHRILIVDDAAFMRMMIKDILVKNGFDVVAEASDGAQAVEKFKEHSPDLVTMDITMPEMDGITALKEIKQIDPQAKIIM
CSAMGQQSMVIDAIQAGAKDFIVKPFQADRVLEAINKTLS

Nucleotide


Download         Length: 363 bp        

>NTDB_id=945137 ACFW98_RS02050 WP_003154238.1 427554..427916(+) (pilH) [Bacillus amyloliquefaciens strain JP5008]
ATGGCACACAGAATTTTAATTGTAGACGATGCAGCATTTATGAGAATGATGATCAAAGATATTTTAGTGAAGAACGGTTT
TGACGTTGTAGCTGAAGCTTCAGACGGAGCGCAGGCAGTCGAAAAATTCAAAGAGCATTCCCCCGACCTTGTGACAATGG
ATATTACAATGCCTGAAATGGACGGGATTACAGCTTTAAAGGAAATTAAACAAATCGATCCGCAGGCGAAAATCATCATG
TGTTCGGCTATGGGCCAGCAGTCAATGGTTATTGACGCGATTCAAGCCGGCGCAAAAGACTTTATCGTGAAACCGTTCCA
AGCGGACCGCGTGCTTGAAGCTATTAATAAAACATTAAGCTAA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(5-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A150FA43

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

38.983

98.333

0.383