Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexB   Type   Machinery gene
Locus tag   ACFW99_RS08785 Genome accession   NZ_CP170651
Coordinates   1752703..1754577 (+) Length   624 a.a.
NCBI ID   WP_192452986.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1747703..1759577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS08770 (ACFW99_08770) ymcA 1748752..1749183 (+) 432 WP_003154113.1 RicAFT regulatory complex protein RicA family protein Regulator
  ACFW99_RS08775 (ACFW99_08775) cotE 1749438..1749983 (+) 546 WP_003154111.1 outer spore coat protein CotE -
  ACFW99_RS08780 (ACFW99_08780) hexA 1750102..1752687 (+) 2586 WP_033574833.1 DNA mismatch repair protein MutS Machinery gene
  ACFW99_RS08785 (ACFW99_08785) hexB 1752703..1754577 (+) 1875 WP_192452986.1 DNA mismatch repair endonuclease MutL Machinery gene
  ACFW99_RS08790 (ACFW99_08790) - 1754773..1755246 (+) 474 WP_060675479.1 tyrosine-type recombinase/integrase -
  ACFW99_RS08795 (ACFW99_08795) - 1755707..1756345 (+) 639 WP_060675480.1 hypothetical protein -
  ACFW99_RS08800 (ACFW99_08800) - 1756421..1756786 (+) 366 WP_060675481.1 HNH endonuclease -
  ACFW99_RS08805 (ACFW99_08805) - 1757072..1758219 (+) 1148 WP_155721238.1 IS3 family transposase -
  ACFW99_RS08810 (ACFW99_08810) - 1758290..1759105 (-) 816 WP_060674177.1 hypothetical protein -

Sequence

Protein


Download         Length: 624 a.a.        Molecular weight: 70194.41 Da        Isoelectric Point: 5.8551

>NTDB_id=944742 ACFW99_RS08785 WP_192452986.1 1752703..1754577(+) (hexB) [Bacillus amyloliquefaciens strain JP5025]
MAKVIQLSDELSNKIAAGEVVERPASVVKELVENAIDANSTVIEIDIEEAGLSSIRVLDNGEGMETDDCKRAFRRHATSK
IKDENDLFRVRTLGFRGEALPSIASVSHLEIKTSIGEGAGTHLILQGGNIISEQKSSSRKGTEIIVTNLFFNTPARLKYM
KTVHTELGNITDVVNRIALAHPEVSIRLRHQGKNLLQTNGNGDVRHVLAAIYGTAVAKKMIPLHVSSLDFEVKGYIALPE
ITRASRNYMSSVINGRYIKNFPLVKAVHEGYHTLLPIGRHPITFIEITMDPILVDVNVHPSKLEVRLSKETELHELIRDG
IKEVFQKQRLIPSAQLPKKSAPAPAKNEQQFMTFEESAPERKLPEKTPEPSYSPMKLSSVVKEPEPVIIEEEILPNEADH
DVPPEIGMPEPEAPVSDTPEPEAVAEEIREERVPVMYPIGQMHGTYILAQNENGLYIIDQHAAQERIKYEYFREKVGQVE
PEVQDMIVPLTFHYAANEALIIDQHQHELESVGVFLEAFGTNSYIVRCHPAWFPKGEEAELIEEIIQQVLDAKQIDIKKL
REEAAIMMSCKGSIKANRHLRNDEIKALLDDLRRTTDPFTCPHGRPIIIHHSTYEMEKMFKRVM

Nucleotide


Download         Length: 1875 bp        

>NTDB_id=944742 ACFW99_RS08785 WP_192452986.1 1752703..1754577(+) (hexB) [Bacillus amyloliquefaciens strain JP5025]
TTGGCAAAAGTCATCCAGCTCTCAGATGAACTTTCAAATAAAATAGCGGCCGGCGAGGTGGTCGAACGGCCTGCCTCAGT
CGTTAAAGAATTGGTAGAAAACGCCATCGACGCAAACAGCACAGTCATTGAAATCGACATAGAGGAAGCGGGGCTTTCGT
CGATTCGGGTGCTCGATAACGGCGAAGGTATGGAAACCGATGATTGCAAGCGGGCTTTCCGCCGCCACGCGACCAGTAAA
ATCAAAGATGAAAACGACCTGTTCCGGGTGAGAACACTCGGTTTCCGCGGAGAAGCTTTGCCAAGTATCGCGTCTGTTTC
TCATCTGGAAATCAAAACGAGCATCGGCGAGGGAGCGGGAACCCATCTCATTCTTCAGGGCGGGAATATCATTTCTGAAC
AGAAATCCTCCAGCAGAAAAGGAACGGAGATTATCGTTACCAACCTGTTTTTCAATACACCCGCCAGATTGAAATATATG
AAAACCGTGCATACGGAGCTCGGCAATATTACGGATGTTGTCAACCGGATCGCTCTTGCGCATCCGGAAGTATCGATTCG
TCTCCGTCATCAGGGGAAAAATCTGCTGCAGACAAACGGTAACGGGGACGTGCGCCATGTGCTGGCCGCAATTTACGGGA
CGGCGGTTGCGAAAAAAATGATACCGCTCCATGTCAGCTCGCTTGATTTTGAAGTCAAGGGCTACATTGCGCTTCCGGAA
ATCACGCGTGCATCCAGAAACTACATGTCATCTGTCATTAACGGAAGATACATTAAGAATTTCCCGCTTGTAAAAGCAGT
GCATGAGGGCTATCACACACTTCTGCCGATCGGCCGCCATCCGATTACATTTATCGAAATTACGATGGACCCGATTTTGG
TCGATGTCAATGTCCATCCGTCGAAGCTTGAAGTCCGCCTCAGCAAAGAGACGGAGCTGCATGAGCTGATCCGTGACGGG
ATAAAGGAAGTGTTTCAAAAGCAGCGGCTTATTCCGAGCGCCCAGCTCCCGAAAAAATCAGCGCCGGCACCGGCCAAAAA
CGAACAGCAATTTATGACATTCGAGGAAAGCGCGCCTGAGCGGAAACTTCCTGAAAAAACGCCGGAGCCGTCTTACTCAC
CAATGAAGCTGAGTTCGGTCGTAAAAGAGCCTGAGCCGGTAATAATAGAAGAAGAGATCCTCCCGAATGAAGCGGATCAC
GATGTGCCGCCGGAGATCGGCATGCCGGAACCGGAAGCGCCTGTTTCCGATACTCCGGAACCGGAAGCTGTCGCTGAAGA
AATACGGGAAGAAAGAGTTCCGGTGATGTATCCGATCGGCCAAATGCACGGGACATATATTTTAGCCCAAAACGAAAACG
GCTTGTATATCATTGACCAGCATGCTGCACAAGAACGGATTAAATACGAATATTTCCGGGAGAAAGTCGGGCAGGTCGAG
CCTGAAGTACAGGATATGATCGTGCCGCTCACCTTTCATTATGCGGCAAATGAGGCGCTGATCATTGACCAGCATCAGCA
TGAGCTTGAAAGCGTCGGAGTATTTTTAGAAGCATTCGGCACGAACAGCTACATCGTTCGCTGCCATCCGGCCTGGTTTC
CAAAAGGGGAAGAAGCTGAACTGATTGAAGAAATCATTCAGCAGGTGCTTGACGCCAAACAGATCGATATTAAGAAACTG
CGGGAGGAAGCGGCCATTATGATGAGCTGCAAAGGCTCAATCAAAGCGAACCGCCATTTGCGAAACGACGAAATCAAAGC
GCTCTTGGATGACCTGCGCCGAACGACAGACCCGTTCACATGCCCGCACGGCCGTCCGATCATAATTCACCACAGCACGT
ATGAGATGGAGAAGATGTTTAAGCGTGTGATGTAG

Domains

Predicted by InterProScan.

MutL C terminal dimerisation domain (MutL_C)

(439-580)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c_3)

(25-111)

DNA mismatch repair protein, C-terminal domain (DNA_mis_repair)

(209-325)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexB Streptococcus pneumoniae R6

46.047

100

0.476